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eNOS (phospho Ser1177) antibody

Anti-eNOS (phospho Ser1177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32227
Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX32227

Cat No. GTX32227

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

133 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of eNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nitric Oxide Synthase 3,Ecnos,Enos,Nos3

Cellular Localization

Caveola,Cell membrane,Cytoplasm,Cytoskeleton,Golgi apparatus,Membrane

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES
Anti-eNOS (phospho Ser1177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32227

GTX32227 ICC/IF Image

ICC/IF analysis of formalin-fixed HUVEC cells using GTX32227 eNOS (phospho Ser1177) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-eNOS (phospho Ser1177) antibody used in Western Blot (WB). GTX32227

GTX32227 WB Image

WB analysis of A549 (A), HUVEC (B), rat brain (C) whole cell lysates using GTX32227 eNOS (phospho Ser1177) antibody.