GeneTex
United States (US)

eRF1 antibody

GTX108271_39778_20160421_WB_M_19011507_777.jpg
GTX108271_39778_IHC_19011507_177.jpg
GTX108271_39778_20141231_IHC_M_2_19011507_888.jpg
GTX108271_39778_20141231_IHC_M_19011507_810.jpg
GTX108271_39778_20160421_WB_R_19011507_751.jpg
GTX108271_39778_20150410_IFA_19011507_821.jpg
GTX108271_39778_20150410_IFA_2_19011507_415.jpg

Cat No. GTX108271

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Rabbit, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human eRF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Termination Factor 1,D5S1995,Erf,Erf1,Rf1,Sup45L1,Tb3-1,Etf1

Cellular Localization

Cytoplasm

Background

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Database

DATA IMAGES
GTX108271_39778_20160421_WB_M_19011507_777.jpg

GTX108271 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eRF1 antibody (GTX108271) diluted at 1:500.

GTX108271_39778_IHC_19011507_177.jpg

GTX108271 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using eRF1(GTX108271) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108271_39778_20141231_IHC_M_2_19011507_888.jpg

GTX108271 IHC-P Image

eRF1 antibody detects eRF1 protein at cytoplasm on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
eRF1 antibody (GTX108271) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108271_39778_20141231_IHC_M_19011507_810.jpg

GTX108271 IHC-P Image

eRF1 antibody detects eRF1 protein at cytoplasm on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
eRF1 antibody (GTX108271) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108271_39778_20160421_WB_R_19011507_751.jpg

GTX108271 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eRF1 antibody (GTX108271) diluted at 1:500.

GTX108271_39778_20150410_IFA_19011507_821.jpg

GTX108271 ICC/IF Image

eRF1 antibody detects eRF1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eRF1 protein stained by eRF1 antibody (GTX108271) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX108271_39778_20150410_IFA_2_19011507_415.jpg

GTX108271 ICC/IF Image

eRF1 antibody detects eRF1 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eRF1 protein stained by eRF1 antibody (GTX108271) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169