PRODUCT
Product Description
Minicircle DNA for iPSC Production + 25 μL each of Nanog, SOX2, OCT3/4 and LIN28A antibodies. Easy iPSC is a simple non-viral solution for reprogramming terminally differentiated cells into pluripotent stem cells. One simple transfection delivers all four transcription factors required. This specially designed minicircle DNA plasmid is devoid of any bacterial replication sequences that would induce endogenous silencing mechanisms, significantly enhancing the transfection efficiency. The vector includes a GFP reporter to easily identify and facilitate sorting of pluripotent cells.
This kit includes the following:
GTX400001 - easy iPSC (100 μL)
GTX100622 - OCT3/4 antibody (25 μL)
GTX101507 - SOX2 antibody (25 μL)
GTX100863 - NANOG antibody (25 μL)
GTX121923 - LIN28A antibody (25 μL)
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
TE Buffer
Storage
Store at -20ºC. Maintain under sterile conditions
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
minicircle DNA, easy-iPSC, easyiPSC, iPSC Minicircle, easy iPSC
DATA IMAGES
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GTX400002 Image
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GTX400002 Image
easy iPSC's minicircle technology enable higher transfection efficiency and maintains expression at higher levels longer than transfection with plasmid DNA.
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GTX400002 RT-PCR Image
RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400002 RT-PCR Image
easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400002 ICC/IF Image
hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400002 Image
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GTX400002 Image
Embryoid bodies derived from easy iPSC-transfected hASC can be differentiated into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400002 Image
Viruses cause transgenes to permanently integrate into the genome and risk insertional mutagenesis. easy iPSC allows for cells to be reprogrammed without integrating into the host cell genome. The southern blot shows multiple gene copies in virally-transduced cells.
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