easy iPSC + Antibody Sample Kit

Cat. No. GTX400002

Application	ICC/IF, RT-PCR
Reactivity	Human, Mouse
Package	1 kit ($835)

See all easy iPSC products

Datasheet

PRODUCT

Summary

Minicircle DNA for iPSC Production + 25 μL each of Nanog, SOX2, OCT3/4 and LIN28A antibodies. Easy iPSC is a simple non-viral solution for reprogramming terminally differentiated cells into pluripotent stem cells. One simple transfection delivers all four transcription factors required. This specially designed minicircle DNA plasmid is devoid of any bacterial replication sequences that would induce endogenous silencing mechanisms, significantly enhancing the transfection efficiency. The vector includes a GFP reporter to easily identify and facilitate sorting of pluripotent cells.
This kit includes the following:
GTX400001 - easy iPSC (100 μL)
GTX100622 - OCT3/4 antibody (25 μL)
GTX101507 - SOX2 antibody (25 μL)
GTX100863 - NANOG antibody (25 μL)
GTX121923 - LIN28A antibody (25 μL)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	Assay dependent
RT-PCR	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

TE Buffer

Preservative

No Preservative

Storage

Store at -20ºC. Maintain under sterile conditions.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

GTX400002 Image

GTX400002 RT-PCR Image

easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).

GTX400002 RT-PCR Image

RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).

GTX400002 Image

GTX400002 ICC/IF Image

hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3).

REFERENCE

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REVIEW

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Package

List Price ($)

1 kit

$ 835

Qty

easy iPSC + Antibody Sample Kit

Cat. No. GTX400002

Application

Reactivity

Summary

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Note

DATA IMAGES

GTX400002 Image

GTX400002 Image

GTX400002 Image

GTX400002 RT-PCR Image

GTX400002 RT-PCR Image

GTX400002 Image

GTX400002 Image

GTX400002 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.