GeneTex
United States (US)

easy iPSC + Antibody Sample Kit

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Cat No. GTX400002

Application ICC/IF, RT-PCR
Reactivity Human, Mouse
PRODUCT

Product Description

Minicircle DNA for iPSC Production + 25 μL each of Nanog, SOX2, OCT3/4 and LIN28A antibodies. Easy iPSC is a simple non-viral solution for reprogramming terminally differentiated cells into pluripotent stem cells. One simple transfection delivers all four transcription factors required. This specially designed minicircle DNA plasmid is devoid of any bacterial replication sequences that would induce endogenous silencing mechanisms, significantly enhancing the transfection efficiency. The vector includes a GFP reporter to easily identify and facilitate sorting of pluripotent cells.
This kit includes the following:
GTX400001 - easy iPSC (100 μL)
GTX100622 - OCT3/4 antibody (25 μL)
GTX101507 - SOX2 antibody (25 μL)
GTX100863 - NANOG antibody (25 μL)
GTX121923 - LIN28A antibody (25 μL)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
RT-PCR Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

TE Buffer

Storage

Store at -20ºC. Maintain under sterile conditions

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

minicircle DNA, easy-iPSC, easyiPSC, iPSC Minicircle, easy iPSC
DATA IMAGES
GTX400002_20180309_1_18121410_850.jpg

GTX400002 Image


GTX400002_20180309_2_18121410_761.jpg

GTX400002 Image

easy iPSC's minicircle technology enable higher transfection efficiency and maintains expression at higher levels longer than transfection with plasmid DNA.

GTX400002_easy_iPSC_chart320110607_19012407_755.jpg

GTX400002 RT-PCR Image

RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).

easy_iPSC_chart220110607_18121410_120.jpg

GTX400002 RT-PCR Image

easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).

easy_iPSC_grids20110607_18121410_371.jpg

GTX400002 ICC/IF Image

hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3).

easy_iPSC20110607_18121410_534.jpg

GTX400002 Image


GTX400002_easy_iPSC_Chart420110607_19012407_663.jpg

GTX400002 Image

Embryoid bodies derived from easy iPSC-transfected hASC can be differentiated into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).

GTX400002_20180309_3_18121410_487.jpg

GTX400002 Image

Viruses cause transgenes to permanently integrate into the genome and risk insertional mutagenesis. easy iPSC allows for cells to be reprogrammed without integrating into the host cell genome. The southern blot shows multiple gene copies in virally-transduced cells.

Package List Price ($)
$ 835