PRODUCT
Summary
Minicircle DNA for iPSC Production + 25 μL each of Nanog, SOX2, OCT3/4 and LIN28A antibodies. Easy iPSC is a simple non-viral solution for reprogramming terminally differentiated cells into pluripotent stem cells. One simple transfection delivers all four transcription factors required. This specially designed minicircle DNA plasmid is devoid of any bacterial replication sequences that would induce endogenous silencing mechanisms, significantly enhancing the transfection efficiency. The vector includes a GFP reporter to easily identify and facilitate sorting of pluripotent cells.
This kit includes the following:
GTX400001 - easy iPSC (100 μL)
GTX100622 - OCT3/4 antibody (25 μL)
GTX101507 - SOX2 antibody (25 μL)
GTX100863 - NANOG antibody (25 μL)
GTX121923 - LIN28A antibody (25 μL)
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
TE Buffer
Storage
Store at -20ºC. Maintain under sterile conditions
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
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GTX400002 ICC/IF Image
hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400002 Image
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GTX400002 Image
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GTX400002 Image
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GTX400002 Image
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GTX400002 Image
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GTX400002 RT-PCR Image
RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400002 RT-PCR Image
easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).
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REFERENCE
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REVIEW
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