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easy iPSC

easy iPSC. GTX400001
easy iPSC. GTX400001
easy iPSC. GTX400001
easy iPSC. GTX400001
easy iPSC. GTX400001
easy iPSC. GTX400001
easy iPSC. GTX400001
easy iPSC. GTX400001

Cat No. GTX400001

Application

 ICC/IF, Cell Culture, RT-PCR

Reactivity

 Human, Mouse
Package
100 μl ($595)
See all easy iPSC products
PRODUCT

Summary

Minicircle DNA for iPSC Production. Easy iPSC is a simple non-viral solution for reprogramming terminally differentiated cells into pluripotent stem cells. One simple transfection delivers all four transcription factors required. This specially designed minicircle DNA plasmid is devoid of any bacterial replication sequences that would induce endogenous silencing mechanisms, significantly enhancing the transfection efficiency. The vector includes a GFP reporter to easily identify and facilitate sorting of pluripotent cells.

APPLICATION

Application Note

This protocol has been optimized for use in human adipose stem cells (hASC). Use in other cell types, such as fibroblasts, will require optimization by the researcher.

PROPERTIES

Form

Liquid

Buffer

0.1X TE Buffer

Preservative

No Preservative

Storage

Store at -20ºC. Maintain under sterile conditions.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

minicircle DNA , easy-iPSC , easyiPSC , iPSC Minicircle , easy iPSC

DATA IMAGES

easy iPSC. GTX400001

GTX400001 RT-PCR Image

easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).

easy iPSC. GTX400001

GTX400001 Image

easy iPSC. GTX400001

GTX400001 Image

easy iPSC. GTX400001

GTX400001 Image

easy iPSC. GTX400001

GTX400001 ICC/IF Image

hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3).

easy iPSC. GTX400001

GTX400001 Image

easy iPSC. GTX400001

GTX400001 Image

easy iPSC. GTX400001

GTX400001 RT-PCR Image

RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).

REFERENCE

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REVIEW

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Package List Price ($)
$ 595