PRODUCT
Product Description
Minicircle DNA for iPSC Production. Easy iPSC is a simple non-viral solution for reprogramming terminally differentiated cells into pluripotent stem cells. One simple transfection delivers all four transcription factors required. This specially designed minicircle DNA plasmid is devoid of any bacterial replication sequences that would induce endogenous silencing mechanisms, significantly enhancing the transfection efficiency. The vector includes a GFP reporter to easily identify and facilitate sorting of pluripotent cells.
APPLICATION
Application Note
This protocol has been optimized for use in human adipose stem cells (hASC). Use in other cell types, such as fibroblasts, will require optimization by the researcher.
PROPERTIES
Form
Liquid
Buffer
0.1X TE Buffer
Storage
Store at -20ºC. Maintain under sterile conditions
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
minicircle DNA, easy-iPSC, easyiPSC, iPSC Minicircle, easy iPSC
DATA IMAGES
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GTX400001 RT-PCR Image
RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400001 RT-PCR Image
easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400001 Image
Embryoid bodies derived from easy iPSC-transfected hASC can be differentiated into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400001 Image
Viruses cause transgenes to permanently integrate into the genome and risk insertional mutagenesis. easy iPSC allows for cells to be reprogrammed without integrating into the host cell genome. The southern blot shows multiple gene copies in virally-transduced cells.
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GTX400001 Image
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GTX400001 ICC/IF Image
hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3).
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GTX400001 Image
easy iPSC's minicircle technology enable higher transfection efficiency and maintains expression at higher levels longer than transfection with plasmid DNA.
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GTX400001 Image
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