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easyTUNEL DNA Fragmentation Assay Kit

easyTUNEL DNA Fragmentation Assay Kit. GTX85582
easyTUNEL DNA Fragmentation Assay Kit. GTX85582

Cat No. GTX85582

Application ICC/IF, FACS
Package
50 assay ($539)
PRODUCT

Product Description

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. GeneTex’s easyTUNEL DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3’-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be observed by fluorescence microscopy or analyzed by flow cytometry.



‧ Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters)

‧ Sample type- Cell culture (adherent and suspension)

‧ Species reactivity- Mammalian

‧ Kit size- 50 assays

‧ Applications- The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.


Features and Benefits

‧ Simple procedure; takes ~ 1 hour

‧ Fast and convenient

‧ The assay is sensitive and stable

‧ The assay offers an one-step labeling of apoptotic cells.



Kit Contents:

Positive Control Cells

Negative Control Cells

Wash Buffer

Reaction Buffer

TdT Enzymes

FITC-dUTP

Rinse Buffer

PI/RNase Staining Buffer

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.
PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TUNEL Assay, Apoptosis Assay, Terminal deoxynucleotidyl transferase dUTP nick end labeling assay, Apoptotic DNA Fragmentation Assay
DATA IMAGES
easyTUNEL DNA Fragmentation Assay Kit. GTX85582

GTX85582 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

easyTUNEL DNA Fragmentation Assay Kit. GTX85582

GTX85582 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

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Package List Price ($)
$ 539