gamma Adaptin antibody [N1N3]

Cat. No. GTX114244

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Wm
Reactivity	Human, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all gamma Adaptin products

Datasheet
Document

View product citations for antibody GTX114244 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-Wm	Assay dependent

Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human gamma Adaptin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167080

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adaptor related protein complex 1 subunit gamma 1 , ADTG , CLAPG1

Cellular Localization

Golgi apparatus , Cytoplasmic vesicle , clathrin-coated vesicle membrane

Background

Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 164 AP1G1
UniProt: O43747 AP1G1

Research Area

DATA IMAGES

GTX114244 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
7.5% SDS PAGE
GTX114244 diluted at 1:1000

GTX114244 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using gamma Adaptin antibody [N1N3] (GTX114244) at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty