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gamma C Crystallin antibody [N1C3]

Anti-gamma C Crystallin antibody [N1C3] used in Western Blot (WB). GTX103004
Anti-gamma C Crystallin antibody [N1C3] used in Western Blot (WB). GTX103004

Cat No. GTX103004

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

A431 , mouse eye

Predict Reactivity

Rat, Bovine, Dog, Guinea pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human gamma C Crystallin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

crystallin gamma C , CCL , CRYG3 , CTRCT2

Background

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq]

Database

DATA IMAGES
Anti-gamma C Crystallin antibody [N1C3] used in Western Blot (WB). GTX103004

GTX103004 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX103004 diluted at 1:1000

Anti-gamma C Crystallin antibody [N1C3] used in Western Blot (WB). GTX103004

GTX103004 WB Image

Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma C Crystallin antibody (GTX103004) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169