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gamma Catenin antibody [C2C3], C-term

Orthogonal
Anti-gamma Catenin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114156
Anti-gamma Catenin antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114156
Anti-gamma Catenin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114156
Anti-gamma Catenin antibody [C2C3], C-term used in Western Blot (WB). GTX114156

Cat. No. GTX114156

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all gamma Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human gamma Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620107

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

junction plakoglobin , CTNNG , DP3 , DPIII , PDGB , PKGB

Background

This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-gamma Catenin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114156

GTX114156 IHC-P Image

gamma Catenin antibody [C2C3], C-term detects gamma Catenin protein at cytosol and membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
gamma Catenin antibody [C2C3], C-term (GTX114156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-gamma Catenin antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114156

GTX114156 IP Image

Gamma Catenin antibody immunoprecipitates Gamma Catenin protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Gamma Catenin protein expressing A431 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Gamma Catenin by 2.5 μg of Gamma Catenin antibody (GTX114156) 7.5% SDS-PAGE The immunoprecipitated Gamma Catenin protein was detected by Gamma Catenin antibody (GTX114156) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-gamma Catenin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114156

GTX114156 IHC-P Image

gamma Catenin antibody [C2C3], C-term detects gamma Catenin protein at cytosol and membrane on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
gamma Catenin antibody [C2C3], C-term (GTX114156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-gamma Catenin antibody [C2C3], C-term used in Western Blot (WB). GTX114156

GTX114156 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with gamma Catenin antibody [C2C3], C-term (GTX114156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169