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gamma Sarcoglycan antibody

Orthogonal
Anti-gamma Sarcoglycan antibody used in Western Blot (WB). GTX117176
Anti-gamma Sarcoglycan antibody used in Western Blot (WB). GTX117176
Anti-gamma Sarcoglycan antibody used in IHC (Paraffin sections) (IHC-P). GTX117176
Anti-gamma Sarcoglycan antibody used in Western Blot (WB). GTX117176

Cat. No. GTX117176

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all gamma Sarcoglycan products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

mouse muscle , rat muscle

Predict Reactivity

Human, Rabbit, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Gamma Sarcoglycan. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885415

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sarcoglycan gamma , 35DAG , A4 , DAGA4 , DMDA , DMDA1 , LGMD2C , LGMDR5 , MAM , SCARMD2 , SCG3 , gamma-SG

Cellular Localization

Cell membrane , sarcolemma; Single-pass type II membrane protein , Cytoplasm , cytoskeleton

Background

This gene encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-gamma Sarcoglycan antibody used in Western Blot (WB). GTX117176

GTX117176 WB Image

Gamma Sarcoglycan antibody detects Gamma Sarcoglycan protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with Gamma Sarcoglycan antibody (GTX117176) diluted by 1:1000.

Anti-gamma Sarcoglycan antibody used in Western Blot (WB). GTX117176

GTX117176 WB Image

Gamma Sarcoglycan antibody detects Gamma Sarcoglycan protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with Gamma Sarcoglycan antibody (GTX117176) diluted by 1:1000.

Anti-gamma Sarcoglycan antibody used in IHC (Paraffin sections) (IHC-P). GTX117176

GTX117176 IHC-P Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2019.PMID: 31689918

Anti-gamma Sarcoglycan antibody used in Western Blot (WB). GTX117176

GTX117176 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2019.PMID: 31689918

REFERENCE

Application Reference

Mamsa H et al. Hum Mol Genet 2022; 31 (5) : 718-732 Sarcospan increases laminin-binding capacity of α-dystroglycan to ameliorate DMD independent of Galgt2.
Application : WB, IHC-Fr
Reactivity : Mouse
Perez-Ortiz AC et al. Int J Mol Sci 2019; 20 (21) Lack of Delta-Sarcoglycan (Sgcd) Results in Retinal Degeneration.
Application : IHC-P
Reactivity : Mouse
Estrada-Mena FJ et al. Neurosci Lett 2017; 658 : 142-149 Effects of (-)-epicatechin on frontal cortex DAPC and dysbindin of the mdx mice.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169