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gamma Synuclein antibody

KO/KD Orthogonal
Anti-gamma Synuclein antibody used in Western Blot (WB). GTX110483
Anti-gamma Synuclein antibody used in Western Blot (WB). GTX110483
Anti-gamma Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110483
Anti-gamma Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX110483
Anti-gamma Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX110483
Anti-gamma Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX110483

Cat. No. GTX110483

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all gamma Synuclein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human gamma Synuclein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951959

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synuclein gamma , BCSG1 , SR

Cellular Localization

Cytoplasm , perinuclear region , Centrosome , Spindle

Background

Summary: This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-gamma Synuclein antibody used in Western Blot (WB). GTX110483

GTX110483 WB Image

KO/KD

Wild-type (WT) and gamma Synuclein knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma Synuclein antibody (GTX110483) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Synuclein antibody used in Western Blot (WB). GTX110483

GTX110483 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma Synuclein antibody (GTX110483) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-gamma Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110483

GTX110483 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using gamma Synuclein (GTX110483) antibody at 1:200 dilution.

Anti-gamma Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX110483

GTX110483 IHC-P Image

gamma Synuclein antibody detects gamma Synuclein protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
gamma Synuclein antibody (GTX110483) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-gamma Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX110483

GTX110483 IHC-Fr Image

The data was published in the journal Mol Neurodegener in 2019.PMID: 31775817

Anti-gamma Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX110483

GTX110483 IHC-Fr Image

The data was published in the journal Sci Rep in 2017.PMID: 29196712

REFERENCE

Application Reference

Hsueh HT et al. Pharmaceutics 2021; 13 (5) Ion-Complex Microcrystal Formulation Provides Sustained Delivery of a Multimodal Kinase Inhibitor from the Subconjunctival Space for Protection of Retinal Ganglion Cells.
Xiao D et al. Sci Adv 2020; 6 (22) : eaaz5858 Generation of self-organized sensory ganglion organoids and retinal ganglion cells from fibroblasts.
Application : IHC-P
Reactivity : Mouse
Welsbie DS et al. Mol Neurodegener 2019; 14 (1) : 44 Targeted disruption of dual leucine zipper kinase and leucine zipper kinase promotes neuronal survival in a model of diffuse traumatic brain injury.
Application : IHC-P
Reactivity : Mouse
Yokoi T et al. Sci Rep 2017; 7 (1) : 16757 Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells.
Application : IHC-Fr
Reactivity : Human
Chintalapudi SR et al. Front Aging Neurosci 2016; 8 : 93 Isolation and Molecular Profiling of Primary Mouse Retinal Ganglion Cells: Comparison of Phenotypes from Healthy and Glaucomatous Retinas.
Application : ICC/IF, IHC
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169