APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
1:50 |
1:200 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 1mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1
Background
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
Database
Research Area
DATA IMAGES
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GTX15794 ICC/IF Image
ICC/IF analysis of HeLa (A) and U2-OS cells (B) using GTX15794 gamma Tubulin antibody [4D11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature
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GTX15794 IHC-P Image
IHC-P analysis of human colon tissue using GTX15794 gamma Tubulin antibody [4D11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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GTX15794 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX15794 gamma Tubulin antibody [4D11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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GTX15794 WB Image
WB analysis of gamma tubulin knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1:1000
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GTX15794 WB Image
WB analysis of 50 ug of various whole cell lysates using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1:1000
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GTX15794 WB Image
WB analysis of membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8) using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1 μg/ml
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REFERENCE
There are currently no references for gamma Tubulin antibody [4D11] (GTX15794). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for gamma Tubulin antibody [4D11] (GTX15794). Be the first to share your experience with this product.