APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:50 |
| 1:200 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 1mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1
Background
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
Database
Research Area
DATA IMAGES
![Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794](/templates/default/js/lightbox/images/loading.gif "Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794")
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GTX15794 ICC/IF Image
ICC/IF analysis of HeLa (A) and U2-OS cells (B) using GTX15794 gamma Tubulin antibody [4D11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature
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GTX15794 IHC-P Image
IHC-P analysis of human colon tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
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GTX15794 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
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GTX15794 WB Image
WB analysis of gamma tubulin knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000
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GTX15794 WB Image
WB analysis of 50 ug of various whole cell lysates using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000
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GTX15794 WB Image
WB analysis of membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1 μg/ml
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REFERENCE
There are currently no references for gamma Tubulin antibody [4D11] (GTX15794). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for gamma Tubulin antibody [4D11] (GTX15794). Be the first to share your experience with this product.
![Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_319_ICC-IF_19011519_995.jpg "Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1045_IHC-P_19011519_878.jpg "Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1044_IHC-P_19011519_720.jpg "Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1539_WB_19011519_828.jpg "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1540_WB_19011519_768.jpg "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1541_WB_19011519_444.jpg "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_319_ICC-IF_19011519_995.jpg "Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1045_IHC-P_19011519_878.jpg "Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1044_IHC-P_19011519_720.jpg "Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1539_WB_19011519_828.jpg "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1540_WB_19011519_768.jpg "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1541_WB_19011519_444.jpg "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")