GeneTex
United States (US)

gamma Tubulin antibody [4D11]

KO/KD
GTX15794_319_ICC-IF_19011519_995.jpg
GTX15794_1045_IHC-P_19011519_878.jpg
GTX15794_1044_IHC-P_19011519_720.jpg
GTX15794_1539_WB_19011519_828.jpg
GTX15794_1540_WB_19011519_768.jpg
GTX15794_1541_WB_19011519_444.jpg

Cat No. GTX15794

Host Mouse
Clonality Monoclonal
Clone Name 4D11
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IP, IHC
Reactivity Human, Mouse, Rat, Primate
See all gamma Tubulin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50
IHC-P 1:200
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CDCBM4,GCP-1,TUBG,TUBGCP1

Cellular Localization

Centrosome,Cytoplasm,Cytoskeleton,Microtubule organizing center

Background

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
DATA IMAGES
GTX15794_319_ICC-IF_19011519_995.jpg

GTX15794 ICC/IF Image

ICC/IF analysis of HeLa (A) and U2-OS cells (B) using GTX15794 gamma Tubulin antibody [4D11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature
GTX15794_1045_IHC-P_19011519_878.jpg

GTX15794 IHC-P Image

IHC-P analysis of human colon tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
GTX15794_1044_IHC-P_19011519_720.jpg

GTX15794 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
GTX15794_1539_WB_19011519_828.jpg

GTX15794 WB Image

KO/KD

WB analysis of gamma tubulin knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000
GTX15794_1540_WB_19011519_768.jpg

GTX15794 WB Image

WB analysis of 50 ug of various whole cell lysates using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000
GTX15794_1541_WB_19011519_444.jpg

GTX15794 WB Image

WB analysis of membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1 µg/ml
Package List Price ($)
$ 319