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gamma Tubulin antibody [GT4511]

Anti-gamma Tubulin antibody [GT4511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629704
Anti-gamma Tubulin antibody [GT4511] used in Western Blot (WB). GTX629704
Anti-gamma Tubulin antibody [GT4511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629704
Anti-gamma Tubulin antibody [GT4511] used in Western Blot (WB). GTX629704
Anti-gamma Tubulin antibody [GT4511] used in Western Blot (WB). GTX629704

Cat No. GTX629704

Host

Mouse

Clonality

Monoclonal

Clone Name

GT4511

Isotype

IgG2b

Application

WB, ICC/IF, IHC

Reactivity

Human, Mouse, Rat, Drosophila
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , Neuro2A , C8D30 , NIH-3T3 , BCL-1 , C2C12 , PC-12 , Rat-2 , *mouse testis ,

Predict Reactivity

Zebrafish, Bovine, Dog, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human gamma Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888139

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Centrosome

Background

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-gamma Tubulin antibody [GT4511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629704

GTX629704 ICC/IF Image

gamma Tubulin antibody [GT4511] detects gamma Tubulin protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: gamma Tubulin stained by gamma Tubulin antibody [GT4511] (GTX629704) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-gamma Tubulin antibody [GT4511] used in Western Blot (WB). GTX629704

GTX629704 WB Image

gamma Tubulin antibody detects gamma Tubulin protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10 % SDS-PAGE
gamma Tubulin antibody (GTX629704) dilution: 1:500

Anti-gamma Tubulin antibody [GT4511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629704

GTX629704 ICC/IF Image

TUBA1B antibody detects TUBG1 protein at Cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min.
Green: TUBG1 protein stained by TUBA1B antibody (GTX629704) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-gamma Tubulin antibody [GT4511] used in Western Blot (WB). GTX629704

GTX629704 WB Image

gamma Tubulin antibody detects gamma Tubulin protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
10 % SDS-PAGE
gamma Tubulin antibody (GTX629704) dilution: 1:500

Anti-gamma Tubulin antibody [GT4511] used in Western Blot (WB). GTX629704

GTX629704 WB Image

gamma Tubulin antibody [GT4511] detects gamma Tubulin protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
gamma Tubulin antibody [GT4511] (GTX629704) dilution: 1:1000

REFERENCE

REVIEW

gamma Tubulin antibody [GT4511] Cat No. GTX629704

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 27-Aug-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse testis
Amount used : 20μg
Blocking : 5% milk/TBST, 25°C, 2Hr
Primary Antibody : 1:2000, 4°C, 12Hr
SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations