gamma Tubulin antibody [GT4511]

Name: gamma Tubulin antibody [GT4511]
Brand: GeneTex
SKU: GTX629704
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-gamma Tubulin antibody [GT4511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629704

Anti-gamma Tubulin antibody [GT4511] used in Western Blot (WB). GTX629704

Cat. No. GTX629704

Reference 4

Host	Mouse
Clonality	Monoclonal
Clone Name	GT4511
Isotype	IgG2b
Application	WB, ICC/IF, IHC
Reactivity	Human, Mouse, Rat, Drosophila
Package	100 μl ($399), 25 μl ($169)

See all gamma Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC	Assay dependent

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , BCL-1 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Zebrafish, Bovine, Dog, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human gamma Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888139

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Centrosome

Background

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq]

Research Area

DATA IMAGES

GTX629704 ICC/IF Image

gamma Tubulin antibody [GT4511] detects gamma Tubulin protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: gamma Tubulin stained by gamma Tubulin antibody [GT4511] (GTX629704) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX629704 WB Image

gamma Tubulin antibody detects gamma Tubulin protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10 % SDS-PAGE
gamma Tubulin antibody (GTX629704) dilution: 1:500

GTX629704 ICC/IF Image

TUBA1B antibody detects TUBG1 protein at Cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min.
Green: TUBG1 protein stained by TUBA1B antibody (GTX629704) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX629704 WB Image

gamma Tubulin antibody detects gamma Tubulin protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
10 % SDS-PAGE
gamma Tubulin antibody (GTX629704) dilution: 1:500

GTX629704 WB Image

gamma Tubulin antibody [GT4511] detects gamma Tubulin protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
gamma Tubulin antibody [GT4511] (GTX629704) dilution: 1:1000

REFERENCE

Application Reference

Konno T et al. J Histochem Cytochem 2019; : 22155419886263 Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells.

Application : ICC/IF

Reactivity : Human

Cheng HW et al. Life Sci Alliance 2019; 2 (1) Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration.

Application : ICC/IF

Reactivity : Human

Nichole M. Owen et al. 0 2015; PARP inhibitor : abrogation of chromosomal radial-induced mitotic cell death and induction of multipolar division via loss of centrosome integrity.

Application : ICC/IF

Reactivity : Human

Okumura T et al. PLoS One 2014; 9 (2) : e89387 βν integrin inhibits chronic and high level activation of JNK to repress senescence phenotypes in Drosophila adult midgut.

Application : IHC

Reactivity : Drosophila

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