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gamma Tubulin antibody [GTU-88]

Anti-gamma Tubulin antibody [GTU-88] used in Western Blot (WB). GTX11316
Anti-gamma Tubulin antibody [GTU-88] used in Western Blot (WB). GTX11316
Anti-gamma Tubulin antibody [GTU-88] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11316

Cat No. GTX11316

Host

Mouse

Clonality

Monoclonal

Clone Name

GTU-88

Isotype

IgG1

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat, Drosophila, Dog, Hamster, Chicken, Monkey, Xenopus
Package
100 μl ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:10,000
ICC/IF 1:5,000-1:10,000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

chicken fibroblast, HeLa

Product Note

Monoclonal Anti-gamma-Tubulin recognizes an epitope located in the N-terminal amino acids 38-53 of gamma-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus gamma-tubulin.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic γ-tubulin peptide (amino acids 38-53), conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384215

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1

Background

The protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.

Database

Research Area

DATA IMAGES

Anti-gamma Tubulin antibody [GTU-88] used in Western Blot (WB). GTX11316

GTX11316 WB Image

WB analysis of chicken fibroblasts using GTX11316 gamma Tubulin antibody [GTU-88].
Lane A : Antibody dilution 1:20,000
Lane B : Negative control (secondary antibody only)

Anti-gamma Tubulin antibody [GTU-88] used in Western Blot (WB). GTX11316

GTX11316 WB Image

WB analysis of (1) HeLa (2) U87 (3) COS7 (4) P19 (5) rat2 (6) CHO (7) MDBK (8) MDCK cell lysate using GTX11316 gamma Tubulin antibody [GTU-88] at 1:10,000.

Anti-gamma Tubulin antibody [GTU-88] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11316

GTX11316 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX11316 gamma Tubulin antibody [GTU-88] at 1:5,000. Cells were fixed and permeabilized with methanol followed by acetone.

REFERENCE

REVIEW

gamma Tubulin antibody [GTU-88] Cat No. GTX11316

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cells in 2020. PMID: 32178256
SDS
Sodium Azide.pdf
Package List Price ($)
$ 389