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gamma Tubulin antibody [HL1175]

Recombinant antibody
Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636480

Cat. No. GTX636480

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1175

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all gamma Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Predict Reactivity

Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human gamma Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909990

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin gamma 1 , tubulin gamma 2 , CDCBM4 , GCP-1 , TUBG , TUBGCP1

Database

Research Area

DATA IMAGES

Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480

GTX636480 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480

GTX636480 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480

GTX636480 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Tubulin antibody [HL1175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636480

GTX636480 ICC/IF Image

gamma Tubulin antibody [HL1175] detects gamma Tubulin protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: gamma Tubulin stained by gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:2500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-gamma Tubulin antibody [HL1175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636480

GTX636480 ICC/IF Image

gamma Tubulin antibody [HL1175] detects gamma Tubulin protein at centrosome and mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: gamma Tubulin stained by gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:2500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations