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gamma Tubulin antibody [HL1175]

Recombinant antibody
Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636480
Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480

Cat. No. GTX636480

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1175

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Rabbit, Drosophila, Cat, Dog, Hamster, African green monkey
Package
100 μl ($399),
25 μl ($169)
See all gamma Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Predict Reactivity

Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human gamma Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909990

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin gamma 1 , tubulin gamma 2 , CDCBM4 , GCP-1 , TUBG , TUBGCP1

Database

Research Area

DATA IMAGES

Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480

GTX636480 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480

GTX636480 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480

GTX636480 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480

GTX636480 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Tubulin antibody [HL1175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636480

GTX636480 ICC/IF Image

gamma Tubulin antibody [HL1175] detects gamma Tubulin protein at centrosome and mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: gamma Tubulin stained by gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:2500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-gamma Tubulin antibody [HL1175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636480

GTX636480 ICC/IF Image

gamma Tubulin antibody [HL1175] detects gamma Tubulin protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: gamma Tubulin stained by gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:2500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-gamma Tubulin antibody [HL1175] used in Western Blot (WB). GTX636480

GTX636480 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (GTX636480) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sun K et al. iScience 2023; 26 (11) : 108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations