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gamma Tubulin antibody [TU-30]

Overexpression
Anti-gamma Tubulin antibody [TU-30] used in Western Blot (WB). GTX79861
Anti-gamma Tubulin antibody [TU-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79861
Anti-gamma Tubulin antibody [TU-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79861
Anti-gamma Tubulin antibody [TU-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79861

Cat No. GTX79861

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TU-30

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Bovine, Chicken, Pig, Protozoa, Plant
Package
100 μg ($379)
See all gamma Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Product Note

This antibody recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin. The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-terminal peptide of gamma-tubulin counjugated to KLH.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11173970

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDCBM4 , GCP1 , TUBG , TUBG1 , TUBGCP1 , tubulin gamma 1

Cellular Localization

Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome

Background

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-gamma Tubulin antibody [TU-30] used in Western Blot (WB). GTX79861

GTX79861 WB Image

Overexpression

WB analysis of SH-SY5Y cells overexpressing TagRFP-tagged or GST-tagged human γ-tubulin 1 (γ-Tb1) or γ-tubulin 2 (γ-Tb2) using GTX79861 gamma Tubulin antibody [TU-30].

Anti-gamma Tubulin antibody [TU-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79861

GTX79861 ICC/IF Image

ICC/IF analysis of mouse fibroblasts using GTX79861 gamma Tubulin antibody [TU-30].
Red : Primary antibody
Blue : DAPI

Anti-gamma Tubulin antibody [TU-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79861

GTX79861 ICC/IF Image

ICC/IF analysis of P19X1 cells using GTX79861 gamma Tubulin antibody [TU-30].
Red : Primary antibody
Blue : DAPI

Anti-gamma Tubulin antibody [TU-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79861

GTX79861 ICC/IF Image

ICC/IF analysis of 3T3 cells (Panel A : metaphase ; Panel B : anaphase ; Panel C : telophase) using GTX79861 gamma Tubulin antibody [TU-30].
Green : alpha Tubulin antibody
Red : Primary antibody
Blue : DAPI

REFERENCE

Application Reference

Yu H et al. Histochem Cell Biol 2020; (Epub) Phosphorylation of keratin 18 serine 52 regulates mother-daughter centriole engagement and microtubule nucleation by cell cycle-dependent accumulation at the centriole.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379