APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1,000 |
1:5,000 |
Not tested in other applications.
Calculated MW
Positive Control
chicken fibroblast, A431
Product Note
Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine).
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).
Purification
IgG fraction
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1
Background
The protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.
Database
Research Area
DATA IMAGES
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GTX11317 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX11317 gamma Tubulin antibody at 1:500(green)..Actin staining with phalloidin rhodamine conjugate(red). Nuclear staining with DAPI(blue).
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GTX11317 WB Image
WB analysis of (1) A431 (2) HeLa (3) Jurkat (4) C2C12 (5) rat2 (6) U87 (7) NIH3T3 (8) CHO cells using GTX11317 gamma Tubulin antibody at 1:1,000.
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GTX11317 ICC/IF Image
ICC/IF analysis of Rat2 cells using GTX11317 gamma Tubulin antibody at 1:1500(green) with DAPI(blue).
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GTX11317 ICC/IF Image
ICC/IF analysis of NIH3T3 cells using GTX11317 gamma Tubulin antibody at 1:500 (green).Actin staining with phalloidin rhodamine conjugate(red). Nuclear staining with DAPI(blue).
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REFERENCE
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REVIEW
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