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gamma Tubulin antibody

Anti-gamma Tubulin antibody used in Western Blot (WB). GTX11317
Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11317
Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11317
Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11317

Cat No. GTX11317

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat, Hamster, Chicken
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ICC/IF 1:5,000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

chicken fibroblast, A431

Product Note

Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine).

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1

Background

The protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.

Database

Research Area

DATA IMAGES

Anti-gamma Tubulin antibody used in Western Blot (WB). GTX11317

GTX11317 WB Image

WB analysis of (1) A431 (2) HeLa (3) Jurkat (4) C2C12 (5) rat2 (6) U87 (7) NIH3T3 (8) CHO cells using GTX11317 gamma Tubulin antibody at 1:1,000.

Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11317

GTX11317 ICC/IF Image

ICC/IF analysis of Rat2 cells using GTX11317 gamma Tubulin antibody at 1:1500(green) with DAPI(blue).

Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11317

GTX11317 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX11317 gamma Tubulin antibody at 1:500 (green).Actin staining with phalloidin rhodamine conjugate(red). Nuclear staining with DAPI(blue).

Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11317

GTX11317 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX11317 gamma Tubulin antibody at 1:500(green)..Actin staining with phalloidin rhodamine conjugate(red). Nuclear staining with DAPI(blue).

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379