*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
chicken fibroblast, A431
Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine).
15mM Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1
The protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.