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gamma Tubulin antibody

Anti-gamma Tubulin antibody used in Western Blot (WB). GTX113286
Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113286
Anti-gamma Tubulin antibody used in Western Blot (WB). GTX113286
Anti-gamma Tubulin antibody used in Immunoprecipitation (IP). GTX113286
Anti-gamma Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX113286
Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113286
Anti-gamma Tubulin antibody used in Western Blot (WB). GTX113286

Cat No. GTX113286

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm, IP, IHC (Formalin), IHC

Reactivity

Human, Mouse, Rat, Zebrafish
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
IHC (Formalin) Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

293T , A431 , HeLa , Jurkat , Raji , NCI-H929 , NIH3T3 , rat kidney

Predict Reactivity

Bovine, Dog, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human gamma Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952442

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GCP-1 antibody , TUBG antibody , TUBGCP1 antibody , TUBG1 antibody , gamma-1-tubulin antibody , "tubulin , gamma polypeptide antibody" , gamma-tubulin complex component 1 antibody , tubulin gamma-1 chain antibody , "tubulin , gamma 1 antibody"

Cellular Localization

Centrosome

Background

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-gamma Tubulin antibody used in Western Blot (WB). GTX113286

GTX113286 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody (GTX113286) diluted at 1:1000.

Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113286

GTX113286 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using gamma Tubulin(GTX113286) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-gamma Tubulin antibody used in Western Blot (WB). GTX113286

GTX113286 WB Image

gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. NIH3T3 whole cell extracts (30 μg) and rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody (GTX113286) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Tubulin antibody used in Immunoprecipitation (IP). GTX113286

GTX113286 IP Image

Immunoprecipitation of gamma Tubulin protein from Jurkat whole cell extracts using 5 μg of gamma Tubulin antibody (GTX113286).
Western blot analysis was performed using gamma Tubulin antibody (GTX113286).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-gamma Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX113286

GTX113286 IHC-P Image

gamma Tubulin antibody detects gamma Tubulin protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
gamma Tubulin antibody (GTX113286) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-gamma Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113286

GTX113286 ICC/IF Image

gamma Tubulin antibody detects gamma Tubulin protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: gamma Tubulin stained by gamma Tubulin antibody (GTX113286) diluted at 1:1000.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-gamma Tubulin antibody used in Western Blot (WB). GTX113286

GTX113286 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody (GTX113286) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

gamma Tubulin antibody Cat No. GTX113286

Rating ( Average 4.9 based on 7 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 2 users reviews)
IHC (Formalin)(IHC (Formalin)) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2014.PMID: 25340762
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2014.PMID: 25340762
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 26010089
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 26683461
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019.PMID: 31831743
Date : Anonymous submitted on 06-Aug-2013
Score :
Application Tested : IHC (Formalin)
Sample Species : Zfsh
Sample : Tail from 24 hpf embryo
Blocking : PBS/Triton X 0.5%/BSA 1%/Normal Goat Serum 2%/DMSO 1%, 25°C, 0.5Hr
Fixation : 4% PFA, 4°C, overnightHr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Imaged with Leica SP5 Confocal at 63X.Blue = DAPI, Red = Acetylated Tubulin, Green = Gamma Tubulin(GTX113286)
Date : Anonymous submitted on 24-Oct-2011
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse brain tissue
Blocking : 10% gaot serum in TBS, 25°C, 1Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : 1% Triton X-100/PBS
Primary Antibody : 1:200, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169