hCG beta protein, His tag

Sandwich ELISA detection of recombinant hCG beta protein, His tag (GTX138833-pro) using antibodies as below.

Capture: hCG beta antibody [GT76] (GTX641721) (5 μg/mL)
Detection: HRP-conjugated hCG beta antibody [GT75] (GTX641720) (1 μg/mL)
Please notice that GTX641720 needs to be conjugated to HRP to function as the detection antibody when paired with GTX641721. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant hCG beta protein, His tag (GTX138833-pro) (1.56-100 nM). Coated protein was probed with hCG beta antibody [GT75] (GTX641720) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant hCG beta protein, His tag (GTX138833-pro) (1.56-100 nM). Coated protein was probed with hCG beta antibody [GT76] (GTX641721) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Detection of recombinant hCG beta protein by lateral flow assay using the monoclonal antibody pair.

Capture: hCG beta antibody [GT76] (GTX641721)
Detection: hCG beta antibody [GT75] (GTX641720)

Samples (20 ng) :
1. Running buffer (N)
2. hCG beta protein (GTX138833-pro)

GTX138833-pro Human hCG beta protein, His tag was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.