GeneTex
United States (US)

hnRNP A1 antibody

KO/KD
GTX106208_39764_20180105_WB_KO_watermark_19011506_102.jpg
GTX106208_39764_20141120_IHC_M_2_19011506_201.jpg
GTX106208_WB_19011506_629.jpg
GTX106208_39764_WB_M_brain_19011506_947.jpg
GTX106208_39764_WB_R_19011506_695.jpg
GTX106208_IFA_19011506_440.jpg
GTX106208_39764_20141120_IHC_M_19011506_724.jpg

Cat No. GTX106208

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , H1299 , HeLaS3 , mouse brain , PC-12

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HNRPA1 antibody, MGC102835 antibody, hnRNP A1 antibody, hnRNP-A1 antibody, HNRNPA1 antibody, hnRNP core protein A1-like 3 antibody, hnRNP A1-like 3 antibody, single-strand RNA-binding protein antibody, heterogeneous nuclear ribonucleoprotein A1 antibody, Putative heterogeneous nuclear ribonucleoprotein A1-like 3 antibody, single-strand DNA-binding protein UP1 antibody, heterogeneous nuclear ribonucleoprotein B2 protein antibody, helix-destabilizing protein antibody, heterogeneous nuclear ribonucleoprotein A1B protein antibody, nuclear ribonucleoprotein particle A1 protein antibody, heterogeneous nuclear ribonucleoprotein core protein A1 antibody, hnRNP core protein A1 antibody

Cellular Localization

Nucleus , Cytoplasm

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106208_39764_20180105_WB_KO_watermark_19011506_102.jpg

GTX106208 WB Image

KO/KD

Wild-type (WT) and hnRNP A1 knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 antibody (GTX106208) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX106208_39764_20141120_IHC_M_2_19011506_201.jpg

GTX106208 IHC-P Image

hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
hnRNP A1 antibody (GTX106208) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106208_WB_19011506_629.jpg

GTX106208 WB Image

Sample(30 µg of whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX106208 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106208_39764_WB_M_brain_19011506_947.jpg

GTX106208 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX106208 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106208_39764_WB_R_19011506_695.jpg

GTX106208 WB Image

hnRNP A1 antibody detects HNRNPA1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12% SDS-PAGE
hnRNP A1 antibody (GTX106208) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106208_IFA_19011506_440.jpg

GTX106208 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP A1(GTX106208) antibody at 1:200 dilution.

GTX106208_39764_20141120_IHC_M_19011506_724.jpg

GTX106208 IHC-P Image

hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
hnRNP A1 antibody (GTX106208) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169