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hnRNP A1 antibody

Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
Anti-hnRNP A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106208
Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
Anti-hnRNP A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106208
Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
Anti-hnRNP A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106208

Cat. No. GTX106208

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

H1299 , HeLaS3 , Mouse brain , PC-12 , 293T

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950504

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein A1 , ALS19 , ALS20 , HNRPA1 , HNRPA1L3 , IBMPFD3 , UP 1 , hnRNP A1 , hnRNP-A1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208

GTX106208 WB Image

Wild-type (WT) and hnRNP A1 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 antibody (GTX106208) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208

GTX106208 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX106208 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106208

GTX106208 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP A1(GTX106208) antibody at 1:200 dilution.

Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208

GTX106208 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX106208 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106208

GTX106208 IHC-P Image

hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
hnRNP A1 antibody (GTX106208) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208

GTX106208 WB Image

hnRNP A1 antibody detects HNRNPA1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
hnRNP A1 antibody (GTX106208) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106208

GTX106208 IHC-P Image

hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
hnRNP A1 antibody (GTX106208) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

hnRNP A1 antibody Cat. No. GTX106208

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Feb-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T WT and HNRNPA1 KO cells
Amount used : 30μg
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note :
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169