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hnRNP A2B1 antibody [HL1706]

Recombinant antibody KO/KD
Anti-hnRNP A2B1 antibody [HL1706] used in Western Blot (WB). GTX637305
Anti-hnRNP A2B1 antibody [HL1706] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637305
Anti-hnRNP A2B1 antibody [HL1706] used in IHC (Paraffin sections) (IHC-P). GTX637305
Anti-hnRNP A2B1 antibody [HL1706] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637305
Anti-hnRNP A2B1 antibody [HL1706] used in Western Blot (WB). GTX637305
Anti-hnRNP A2B1 antibody [HL1706] used in Western Blot (WB). GTX637305

Cat. No. GTX637305

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1706

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all hnRNP A2B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein A2/B1 , HNRNPA2 , HNRNPB1 , HNRPA2 , HNRPA2B1 , HNRPB1 , IBMPFD2 , RNPA2 , SNRPB1

Cellular Localization

nucleoplasm,Cytoplasm

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 3181 HNRNPA2B1
  • UniProt: P22626 HNRNPA2B1

Research Area

DATA IMAGES

Anti-hnRNP A2B1 antibody [HL1706] used in Western Blot (WB). GTX637305

GTX637305 WB Image

KO/KD

Wild-type (WT) and HNRNPA2B1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody [HL1706] (GTX637305) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A2B1 antibody [HL1706] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637305

GTX637305 ICC/IF Image

hnRNP A2B1 antibody [HL1706] detects hnRNP A2B1 protein by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A2B1 stained by hnRNP A2B1 antibody [HL1706] (GTX637305) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-hnRNP A2B1 antibody [HL1706] used in IHC (Paraffin sections) (IHC-P). GTX637305

GTX637305 IHC-P Image

hnRNP A2B1 antibody [HL1706] detects hnRNP A2B1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
hnRNP A2B1 stained by hnRNP A2B1 antibody [HL1706] (GTX637305) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP A2B1 antibody [HL1706] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637305

GTX637305 ICC/IF Image

hnRNP A2B1 antibody [HL1706] detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A2B1 stained by hnRNP A2B1 antibody [HL1706] (GTX637305) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-hnRNP A2B1 antibody [HL1706] used in Western Blot (WB). GTX637305

GTX637305 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody [HL1706] (GTX637305) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A2B1 antibody [HL1706] used in Western Blot (WB). GTX637305

GTX637305 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody [HL1706] (GTX637305) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations