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hnRNP A2B1 antibody

KO/KD
Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX114475
Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX114475
Anti-hnRNP A2B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114475
Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX114475
Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114475

Cat. No. GTX114475

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all hnRNP A2B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

HeLa , 293T

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165592

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein A2/B1 , HNRNPA2 , HNRNPB1 , HNRPA2 , HNRPA2B1 , HNRPB1 , IBMPFD2 , RNPA2 , SNRPB1

Cellular Localization

Nucleus , nucleoplasm , Cytoplasm

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq]

Database

  • Gene ID: 3181 HNRNPA2B1
  • UniProt: P22626 HNRNPA2B1

Research Area

DATA IMAGES

Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX114475

GTX114475 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX114475) diluted at 1:2000.

Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX114475

GTX114475 WB Image

KO/KD

Wild-type (WT) and hnRNP A2B1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX114475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A2B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114475

GTX114475 ICC/IF Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A2B1 protein stained by hnRNP A2B1 antibody (GTX114475) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX114475

GTX114475 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX114475 diluted at 1:5000

Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114475

GTX114475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using hnRNP A2B1(GTX114475) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169