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United States (US)

hnRNP A2B1 antibody

GTX117578_40646_20161117_WB_shRNA_watermark_18121410_218.jpg
GTX117578_40646_IHC_18121410_189.jpg
GTX117578_40646_WB_18121410_140.jpg
GTX117578_40646_IFA_18121410_240.jpg

Cat No. GTX117578

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heterogeneous Nuclear Ribonucleoprotein A2/B1,Hnrnpa2,Hnrnpb1,Hnrpa2,Hnrpa2B1,Hnrpb1,Ibmpfd2,Rnpa2,Snrpb1,Hnrnpa2B1

Cellular Localization

Nucleus , nucleoplasm , Cytoplasm

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX117578_40646_20161117_WB_shRNA_watermark_18121410_218.jpg

GTX117578 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX117578) diluted at 1:5000.

GTX117578_40646_IHC_18121410_189.jpg

GTX117578 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using hnRNP A2B1(GTX117578) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX117578_40646_WB_18121410_140.jpg

GTX117578 WB Image

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
GTX117578 diluted at 1:10000

GTX117578_40646_IFA_18121410_240.jpg

GTX117578 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using hnRNP A2B1(GTX117578) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169