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hnRNP A2B1 antibody

Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX127928
Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX127928
Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX127928
Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928
Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928
Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928
Anti-hnRNP A2B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127928
Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928
Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928
Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928
Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928

Cat. No. GTX127928

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2616069

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein A2/B1 , HNRNPA2 , HNRNPB1 , HNRPA2 , HNRPA2B1 , HNRPB1 , IBMPFD2 , RNPA2 , SNRPB1

Cellular Localization

Nucleus , nucleoplasm , Cytoplasm

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq]

Database

  • Gene ID: 3181 HNRNPA2B1
  • UniProt: P22626 HNRNPA2B1

Research Area

DATA IMAGES

Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX127928

GTX127928 WB Image

Wild-type (WT) and hnRNP A2B1 knockout (KO) HEK293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX127928

GTX127928 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A2B1 antibody used in Western Blot (WB). GTX127928

GTX127928 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP A2B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127928

GTX127928 ICC/IF Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at endoplasmic reticulum and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
hnRNP A2B1 antibody (GTX127928) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
hnRNP A2B1 antibody (GTX127928) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-hnRNP A2B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127928

GTX127928 IHC-P Image

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
hnRNP A2B1 stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

hnRNP A2B1 antibody Cat. No. GTX127928

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 29-Jul-2016
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : NSC-34 cells
Blocking : 3% normal goat serum in PBS, 25°C, 1Hr
Fixation : methanol
Primary Antibody : 1:500, 4°C, 18Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169