hnRNP C1/C2 antibody

Cat. No. GTX113463

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all hnRNP C1/C2 products

View product citations for antibody GTX113463 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Raji , Neuro2A , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Rat, Rabbit, Bovine, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 1% BSA, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP C1/C2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037184

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein C (C1/C2) , C1 , C2 , HNRNP , HNRPC , SNRPC

Cellular Localization

Nucleus

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq]

Database

Gene ID: 3183 HNRNPC
UniProt: P07910 HNRNPC

Research Area

DATA IMAGES

GTX113463 IHC-P Image

hnRNP C1/C2 antibody detects hnRNP C1/C2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
hnRNP C1/C2 antibody (GTX113463) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113463 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
12% SDS PAGE
GTX113463 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113463 WB Image

hnRNP C1/C2 antibody detects HNRNPC protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
12% SDS-PAGE
hnRNP C1/C2 antibody (GTX113463) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113463 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using hnRNP C1/C2 (GTX113463) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113463 ICC/IF Image

hnRNP C1/C2 antibody detects hnRNP C1/C2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP C1/C2 protein stained by hnRNP C1/C2 antibody (GTX113463) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX113463 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP C1/C2 antibody (GTX113463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.