hnRNP F antibody [5F5]

Name: hnRNP F antibody [5F5]
Brand: GeneTex
SKU: GTX84356
Price: 519.00 USD

Anti-hnRNP F antibody [5F5] used in IHC (Paraffin sections) (IHC-P). GTX84356

Anti-hnRNP F antibody [5F5] used in Flow cytometry (FACS). GTX84356

Anti-hnRNP F antibody [5F5] used in Western Blot (WB). GTX84356

Anti-hnRNP F antibody [5F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84356

Cat. No. GTX84356

Host	Mouse
Clonality	Monoclonal
Clone Name	5F5
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($519)

See all hnRNP F products

Datasheet
Document

View product citations for antibody GTX84356 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:50-1:100
IHC-P	1:50
FACS	1:100

Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein F , HNRPF , OK/SW-cl.23 , mcs94-1

Cellular Localization

nucleoplasm

Background

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Database

Gene ID: 3185 HNRNPF
UniProt: P52597 HNRNPF

Research Area

DATA IMAGES

GTX84356 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84356 hnRNP F antibody [5F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84356 FACS Image

FACS analysis of HeLa cells using GTX84356 hnRNP F antibody [5F5].
Red : Primary antibody
Blue : Negative control antibody

GTX84356 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX84356 hnRNP F antibody [5F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84356 WB Image

WB analysis of wild type and hnRNP F knock out 293T cell lysate(10μg per lane) using GTX84356 hnRNP F antibody [5F5].
Dilution : 1:500

GTX84356 IHC-P Image

IHC-P analysis of human liver tissue using GTX84356 hnRNP F antibody [5F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84356 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84356 hnRNP F antibody [5F5].

GTX84356 FACS Image

FACS analysis of Jurkat cells using GTX84356 hnRNP F antibody [5F5].
Red : Primary antibody
Blue : Negative control antibody

GTX84356 WB Image

WB analysis of HEK293T cells transfected with hnRNP F plasmid (Right) or empty vector (Left) for 48 hrs using GTX84356 hnRNP F antibody [5F5].
Loading : 5 ug per lane

GTX84356 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with hnRNP F plasmid using GTX84356 hnRNP F antibody [5F5].

GTX84356 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84356 hnRNP F antibody [5F5].
Loading : 10 ug per lane
Dilution : 1:1000

GTX84356 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84356 hnRNP F antibody [5F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84356 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84356 hnRNP F antibody [5F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

hnRNP F antibody [5F5]

Cat. No. GTX84356

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84356 IHC-P Image

GTX84356 FACS Image

GTX84356 IHC-P Image

GTX84356 WB Image

GTX84356 IHC-P Image

GTX84356 ICC/IF Image

GTX84356 FACS Image

GTX84356 WB Image

GTX84356 ICC/IF Image

GTX84356 WB Image

GTX84356 IHC-P Image

GTX84356 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.