GeneTex

  • Country / Location Selection

United States (US)

hnRNP F antibody [N1N3]

KO/KD Orthogonal
Anti-hnRNP F antibody [N1N3] used in Western Blot (WB). GTX114476
Anti-hnRNP F antibody [N1N3] used in Western Blot (WB). GTX114476
Anti-hnRNP F antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114476
Anti-hnRNP F antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114476
Anti-hnRNP F antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114476
Anti-hnRNP F antibody [N1N3] used in Immunoprecipitation (IP). GTX114476

Cat. No. GTX114476

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all hnRNP F products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037186

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein F , HNRPF , OK/SW-cl.23 , mcs94-1

Cellular Localization

Nucleus , nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-hnRNP F antibody [N1N3] used in Western Blot (WB). GTX114476

GTX114476 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] (GTX114476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP F antibody [N1N3] used in Western Blot (WB). GTX114476

GTX114476 WB Image

Orthogonal

HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] (GTX114476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-hnRNP F antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114476

GTX114476 IHC-P Image

hnRNP F antibody [N1N3] detects hnRNP F protein at nucleus on mouse uterus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse uterus.
hnRNP F antibody [N1N3] (GTX114476) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP F antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114476

GTX114476 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft , using hnRNP F (GTX114476) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-hnRNP F antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114476

GTX114476 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using hnRNP F (GTX114476) antibody at 1:200 dilution.

Anti-hnRNP F antibody [N1N3] used in Immunoprecipitation (IP). GTX114476

GTX114476 IP Image

Immunoprecipitation of hnRNP F protein from 293T whole cell extracts using 5 μg of hnRNP F antibody [N1N3] (GTX114476).
Western blot analysis was performed using hnRNP F antibody [N1N3] (GTX114476).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF, IP
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for hnRNP F antibody [N1N3] (GTX114476). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169