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United States (US)

hnRNP F antibody

Anti-hnRNP F antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33243
Anti-hnRNP F antibody used in Western Blot (WB). GTX33243

Cat No. GTX33243

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

46 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPF (NP_001091678.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heterogeneous Nuclear Ribonucleoprotein F,Hnrpf,Ok/Sw-Cl.23,Mcs94-1,Hnrnpf

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-hnRNP F antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33243

GTX33243 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX33243 hnRNP F antibody.
Blue : DAPI

Anti-hnRNP F antibody used in Western Blot (WB). GTX33243

GTX33243 WB Image

WB analysis of various samples using GTX33243 hnRNP F antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329