GeneTex
United States (US)

hnRNP H antibody [N1C1]

KO/KD
GTX102061_40121_20181005_WB_shRNA_watermark_19011506_660.jpg
GTX102061_WB_19011506_670.jpg
GTX102061_40121_WB_19011506_916.jpg
GTX102061_IHC_19011506_212.jpg
GTX102061_40121_WB_M_brain_19011506_147.jpg
GTX102061_40121_20150410_IFA_19011506_419.jpg
GTX102061_40121_20150410_IFA_2_19011506_900.jpg

Cat No. GTX102061

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Raji , NCI-H929 , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP H. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heterogeneous Nuclear Ribonucleoprotein H1,Hnrph,Hnrph1,Hnrnph,Hnrnph1

Cellular Localization

Nucleus , nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype. [provided by RefSeq]

Database

DATA IMAGES
GTX102061_40121_20181005_WB_shRNA_watermark_19011506_660.jpg

GTX102061 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody [N1C1] (GTX102061) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102061_WB_19011506_670.jpg

GTX102061 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
10% SDS PAGE
GTX102061 diluted at 1:1000

GTX102061_40121_WB_19011506_916.jpg

GTX102061 WB Image

hnRNP H antibody detects hnRNP H protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody (GTX102061) diluted by 1:1000.

GTX102061_IHC_19011506_212.jpg

GTX102061 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using hnRNP H(GTX102061) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102061_40121_WB_M_brain_19011506_147.jpg

GTX102061 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102061 diluted at 1:1000

GTX102061_40121_20150410_IFA_19011506_419.jpg

GTX102061 ICC/IF Image

hnRNP H antibody [N1C1] detects hnRNP H protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP H protein stained by hnRNP H antibody [N1C1] (GTX102061) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX102061_40121_20150410_IFA_2_19011506_900.jpg

GTX102061 ICC/IF Image

hnRNP H antibody [N1C1] detects hnRNP H protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP H protein stained by hnRNP H antibody [N1C1] (GTX102061) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169