Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody [N1C1] (GTX102061) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using hnRNP H(GTX102061) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
10% SDS PAGE
GTX102061 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102061 diluted at 1:1000

hnRNP H antibody [N1C1] detects hnRNP H protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP H protein stained by hnRNP H antibody [N1C1] (GTX102061) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

hnRNP H antibody detects hnRNP H protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody (GTX102061) diluted by 1:1000.

hnRNP H antibody [N1C1] detects hnRNP H protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP H protein stained by hnRNP H antibody [N1C1] (GTX102061) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.