GeneTex
United States (US)

hnRNP H2 antibody

GTX106825_41031_20160630_WB_R_heart_19011506_953.jpg
GTX106825_41031_20160616_WB_M_heart_19011506_702.jpg
GTX106825_41031_IHC_M_19011506_475.jpg
GTX106825_41031_IHC_M_2_19011506_664.jpg
GTX106825_41031_20160601_IHC-P_R_19011506_514.jpg
GTX106825_41031_IHC_R_19011506_711.jpg

Cat No. GTX106825

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Rat heart , Mouse heart

Predict Reactivity

Human, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP H2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FTP3 Antibody, HNRNPH2 Antibody, HNRPH2 Antibody, HNRPH' Antibody, heterogeneous nuclear ribonucleoprotein H2 (H') Antibody, hnRNPH' Antibody

Cellular Localization

Nucleus , nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that binds to RNAs. It is very similar to the family member HNRPH1. This gene is thought to be involved in Fabray disease and X-linked agammaglobulinemia phenotype. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

DATA IMAGES
GTX106825_41031_20160630_WB_R_heart_19011506_953.jpg

GTX106825 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H2 antibody (GTX106825) diluted at 1:500.

GTX106825_41031_20160616_WB_M_heart_19011506_702.jpg

GTX106825 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H2 antibody (GTX106825) diluted at 1:500.

GTX106825_41031_IHC_M_19011506_475.jpg

GTX106825 IHC-P Image

hnRNP H2 antibody detects hnRNP H2 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
hnRNP H2 antibody (GTX106825) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106825_41031_IHC_M_2_19011506_664.jpg

GTX106825 IHC-P Image

hnRNP H2 antibody detects hnRNP H2 protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
hnRNP H2 antibody (GTX106825) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106825_41031_20160601_IHC-P_R_19011506_514.jpg

GTX106825 IHC-P Image

hnRNP H2 antibody detects hnRNP H2 protein at cytoplasm and nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
hnRNP H2 antibody (GTX106825) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106825_41031_IHC_R_19011506_711.jpg

GTX106825 IHC-P Image

hnRNP H2 antibody detects hnRNP H2 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
hnRNP H2 antibody (GTX106825) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169