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hnRNP K antibody [3C2]

Anti-hnRNP K antibody [3C2] used in Western Blot (WB). GTX80387

Cat. No. GTX80387

Host

Mouse

Clonality

Monoclonal

Clone Name

3C2

Isotype

IgG2b

Application

WB, ICC/IF, FACS, IP, ELISA, ChIP assay

Reactivity

Human, Mouse, Rat, Bovine, Hamster, Xenopus laevis
Package
50 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
FACS 1μg for 10^6 cells
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Product Note

Human hnRNP K. Detects a band of approximately 65 kDa. Cross reacts with rat, xenopus, bovine & hamster

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified full length native human protein

Purification

Protein A

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein K , AUKS , CSBP , HNRPK , TUNP

Cellular Localization

Cytoplasm , nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-hnRNP K antibody [3C2] used in Western Blot (WB). GTX80387

GTX80387 WB Image

WB analysis of HeLa cell lysate using GTX80387 hnRNP K antibody [3C2].
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399