GeneTex
United States (US)

hnRNP K antibody [F45 P9 C7]

GTX15882_2037_IHC-P_19011519_300.jpg
GTX15882_346_ICC-IF_19011519_167.jpg
GTX15882_1550_WB_19011519_886.jpg

Cat No. GTX15882

Host Mouse
Clonality Monoclonal
Clone Name F45 P9 C7
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse, Rat, Rabbit, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 μg/mL
ICC/IF 5 μg/ml
IHC-P 5 μg/mL
IHC Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, conjugated to Ovalbumin, corresponding to residues S(453) V K Q Y S G K F F(463) of the hnRNP-K protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heterogeneous Nuclear Ribonucleoprotein K,Auks,Csbp,Hnrpk,Tunp,Hnrnpk

Cellular Localization

Cell projection,Cytoplasm,Nucleoplasm,Nucleus,Podosome

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15882_2037_IHC-P_19011519_300.jpg

GTX15882 IHC-P Image

IHC-P analysis of colon carcinoma tissue using GTX15882 hnRNP K antibody [F45 P9 C7].

GTX15882_346_ICC-IF_19011519_167.jpg

GTX15882 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15882 hnRNP K antibody [F45 P9 C7]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 5 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature

GTX15882_1550_WB_19011519_886.jpg

GTX15882 WB Image

WB analysis of whole cell extracts (30 µg lysate) of HeLa (Lane 1), PANC-1 (Lane 2), MCF7 (Lane 3), U-2 OS (Lane 4), U-87 MG (Lane 5), IMR-32 (Lane 6), HT-29 (Lane 7) and tissue extract of Rat Testis (Lane 10) using GTX15882 hnRNP K antibody [F45 P9 C7].
Dilution : 2 µg/ml

Package List Price ($)
$ 319