hnRNP K antibody [F45 P9 C7]

Cat. No. GTX15882

Host	Mouse
Clonality	Monoclonal
Clone Name	F45 P9 C7
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat, Rabbit, Chicken
Package	100 μg ($399)

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Datasheet
Document

View product citations for antibody GTX15882 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	2 μg/mL
ICC/IF	5 μg/ml
IHC-P	5 μg/mL
IHC	Assay dependent

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, conjugated to Ovalbumin, corresponding to residues S(453) V K Q Y S G K F F(463) of the hnRNP-K protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein K , AUKS , CSBP , HNRPK , TUNP

Cellular Localization

Cytoplasm,nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3190 HNRNPK
UniProt: P61978 HNRNPK

Research Area

DATA IMAGES

GTX15882 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15882 hnRNP K antibody [F45 P9 C7]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 5 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

GTX15882 IHC-P Image

IHC-P analysis of colon carcinoma tissue using GTX15882 hnRNP K antibody [F45 P9 C7].

GTX15882 WB Image

WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1), PANC-1 (Lane 2), MCF7 (Lane 3), U-2 OS (Lane 4), U-87 MG (Lane 5), IMR-32 (Lane 6), HT-29 (Lane 7) and tissue extract of Rat Testis (Lane 10) using GTX15882 hnRNP K antibody [F45 P9 C7].
Dilution : 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty