GeneTex
United States (US)

hnRNP K antibody

GTX101815_40527_IFA_19011506_483.jpg
GTX101815_40527_WB_19011506_730.jpg

Cat No. GTX101815

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CSBP antibody, FLJ41122 antibody, HNRPK antibody, TUNP antibody, HNRNPK antibody, dC-stretch binding protein antibody, transformation upregulated nuclear protein antibody, heterogeneous nuclear ribonucleoprotein K antibody

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101815_40527_IFA_19011506_483.jpg

GTX101815 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP K(GTX101815) antibody at 1:500 dilution.

GTX101815_40527_WB_19011506_730.jpg

GTX101815 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX101815 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169