GeneTex
United States (US)

hnRNP L antibody

hnRNP-L-antibody-GTX87723-ICC_IF-1_18121411_625.jpg
hnRNP-L-antibody-GTX87723-WB-1_18121411_715.jpg
hnRNP-L-antibody-GTX87723-IHC-1_18121411_195.jpg

Cat No. GTX87723

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

hnRNP L Antibody detects endogenous levels of total hnRNP L protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human hnRNP L.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heterogeneous Nuclear Ribonucleoprotein L,Hnrpl,P/Okcl.14,Hnrnp-L,Hnrnpl

Cellular Localization

Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Background

This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. L is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes.

Database

DATA IMAGES
hnRNP-L-antibody-GTX87723-ICC_IF-1_18121411_625.jpg

GTX87723 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using hnRNP L Antibody. The picture on the right is treated with the synthesized peptide.

hnRNP-L-antibody-GTX87723-WB-1_18121411_715.jpg

GTX87723 WB Image

Western blot analysis of extracts from HeLa/Jurkat/HuvEc cells, using hnRNP L Antibody. The lane on the right is treated with the synthesized peptide.

hnRNP-L-antibody-GTX87723-IHC-1_18121411_195.jpg

GTX87723 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using hnRNP L Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339