APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 491-730 of human HNRNPM (NP_005959.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
heterogeneous nuclear ribonucleoprotein M , CEAR , HNRNPM4 , HNRPM , HNRPM4 , HTGR1 , NAGR1 , hnRNP M
Cellular Localization
Nucleolus
Background
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
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GTX33245 WB Image
WB analysis of various sample lysates using GTX33245 hnRNP M antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX33245 IHC-P Image
IHC-P analysis of human appendix tissue using GTX33245 hnRNP M antibody.
Dilution : 1:100
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GTX33245 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX33245 hnRNP M antibody.
Dilution : 1:100
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GTX33245 IHC-P Image
IHC-P analysis of human esophageal cancer tissue using GTX33245 hnRNP M antibody.
Dilution : 1:100
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GTX33245 ICC/IF Image
ICC/IF analysis of C6 cells using GTX33245 hnRNP M antibody.
Blue : DAPI
Dilution : 1:100
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GTX33245 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX33245 hnRNP M antibody.
Dilution : 1:100
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GTX33245 IHC-P Image
IHC-P analysis of rat brain tissue using GTX33245 hnRNP M antibody.
Dilution : 1:100
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GTX33245 ICC/IF Image
ICC/IF analysis of L929 cells using GTX33245 hnRNP M antibody.
Blue : DAPI
Dilution : 1:100
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GTX33245 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX33245 hnRNP M antibody.
Blue : DAPI
Dilution : 1:100
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REFERENCE
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REVIEW
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