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hnRNP M1-M4 antibody [1D8]

Anti-hnRNP M1-M4 antibody [1D8] used in IHC (Paraffin sections) (IHC-P). GTX29547
Anti-hnRNP M1-M4 antibody [1D8] used in Western Blot (WB). GTX29547

Cat. No. GTX29547

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Pig
Package
200 μl ($419)
See all hnRNP M1-M4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:10 - 1:500
IHC-P 1:200
IHC-Fr 1:10 - 1:500
IP 1:10 - 1:500
Not tested in other applications.

Product Note

This antibody is specific for all four M proteins of hnRNP (M1, M2, M3 and M4).

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

M19 fusion protein containing full-length human protein. [UniProt# P52272]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogenous nuclear ribonucleoproteins (hnRNPs), play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64kDa subunit of cleavage stimulation factor, which is involved in 3'-end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

Research Area

DATA IMAGES

Anti-hnRNP M1-M4 antibody [1D8] used in IHC (Paraffin sections) (IHC-P). GTX29547

GTX29547 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX29547 HNRNP M1-M4 antibody [1D8].
Dilution : 1:500
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

Anti-hnRNP M1-M4 antibody [1D8] used in Western Blot (WB). GTX29547

GTX29547 WB Image

WB analysis of HeLa cell lysate using GTX29547 HNRNP M1-M4 antibody [1D8].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419