GeneTex
United States (US)

hnRNP M3-M4 antibody [2A6]

GTX30273_1383_WB_18121410_215.jpg
GTX30273_1384_WB_18121410_937.jpg
GTX30273_546_IHC-P_18121410_767.jpg

Cat No. GTX30273

Host Mouse
Clonality Monoclonal
Clone Name 2A6
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat, Rabbit, Bovine, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:10 - 1:500
IHC-P 1:10 - 1:500
IHC-Fr 1:10 - 1:500
IP 1:10 - 1:500
Not tested in other applications.

Specificity/Sensitivity

This antibody is specific for hnRNP M3 and M4 proteins.
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

M19 fusion protein containing full-length human protein. [UniProt# P52272]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogenous nuclear ribonucleoproteins (hnRNPs), play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64kDa subunit of cleavage stimulation factor, which is involved in 3'-end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.
DATA IMAGES
GTX30273_1383_WB_18121410_215.jpg

GTX30273 WB Image

WB analysis of HeLa cell lysate using GTX30273 hnRNP M3-M4 antibody [2A6].

GTX30273_1384_WB_18121410_937.jpg

GTX30273 WB Image

WB analysis of HeLa cell lysate using GTX30273 hnRNP M3-M4 antibody [2A6].

GTX30273_546_IHC-P_18121410_767.jpg

GTX30273 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX30273 hnRNP M3-M4 antibody [2A6].
Dilution : 1:500
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

Package List Price ($)
$ 319