GeneTex
United States (US)

hnRNP R antibody [N1N2], N-term

KO/KD
GTX106526_40807_20180202_WB_KO_watermark_19011506_467.jpg
GTX106526_40807_WB_19011506_718.jpg
GTX106526_40807_20150601_IFA_19011506_950.jpg

Cat No. GTX106526

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human hnRNP R. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ25714 antibody, HNRPR antibody, hnRNP R antibody, hnRNP-R antibody, HNRNPR antibody, heterogeneous nuclear ribonucleoprotein R antibody

Cellular Localization

Nucleus , nucleoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs and also contains a nuclear localization motif. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106526_40807_20180202_WB_KO_watermark_19011506_467.jpg

GTX106526 WB Image

KO/KD

Wild-type (WT) and hnRNP R knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hnRNP R antibody [N1N2], N-term (GTX106526) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX106526_40807_WB_19011506_718.jpg

GTX106526 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX106526 diluted at 1:3000

GTX106526_40807_20150601_IFA_19011506_950.jpg

GTX106526 ICC/IF Image

hnRNP R antibody [N1N2], N-term detects hnRNP R protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP R protein stained by hnRNP R antibody [N1N2], N-term (GTX106526) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169