APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
Observed MW
120 kDa. The observed M.W. is based on the following publication. PMID: 22345668
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human hnRNP U
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heterogeneous nuclear ribonucleoprotein U , EIEE54 , GRIP120 , HNRNPU-AS1 , HNRPU , SAF-A , SAFA , U21.1 , hnRNP U , pp120
Cellular Localization
Nucleus , Nucleus speckle , Cytoplasm
Background
This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14. [provided by RefSeq, Jun 2017]
Database
Research Area
DATA IMAGES
![Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839](/templates/default/js/lightbox/images/loading.gif "Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839")
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GTX02839 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with hnRNP U antibody [GT1242] (GTX02839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22345668
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GTX02839 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with hnRNP U antibody [GT1242] (GTX02839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22345668
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GTX02839 ICC/IF Image
ICC/IF analysis of C6 cells using GTX02839 hnRNP U antibody [GT1242].
Dilution : 1:100
Blue : DAPI for nuclear staining.
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GTX02839 IHC-P Image
IHC-P analysis of rat liver tissue section using GTX02839 hnRNP U antibody [GT1242].
Dilution : 1:100
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GTX02839 IP Image
IP analysis of K562 whole cell lysate using GTX02839 hnRNP U antibody [GT1242].
Total extract : 300μg
IP antibody amount : 3μg
Dilution : 1:1000
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GTX02839 IHC-P Image
IHC-P analysis of mouse liver tissue section using GTX02839 hnRNP U antibody [GT1242].
Dilution : 1:100
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GTX02839 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX02839 hnRNP U antibody [GT1242].
Dilution : 1:100
Blue : DAPI for nuclear staining.
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GTX02839 WB Image
WB analysis of various samples using GTX02839 hnRNP U antibody [GT1242].
Dilution : 1:1000
Loading : 25μg
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GTX02839 IHC-P Image
IHC-P analysis of human thyroid cancer section using GTX02839 hnRNP U antibody [GT1242].
Dilution : 1:100
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GTX02839 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX02839 hnRNP U antibody [GT1242].
Dilution : 1:100
Blue : DAPI for nuclear staining.
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REFERENCE
There are currently no references for hnRNP U antibody [GT1242] (GTX02839). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for hnRNP U antibody [GT1242] (GTX02839). Be the first to share your experience with this product.
![Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839](/upload/website/prouct_img/small/GTX02839/GTX02839_4000000942_20210122_WB_w_23053123_128.webp "Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839](/upload/website/prouct_img/small/GTX02839/GTX02839_4000000942_20210122_WB_2_w_23053123_816.webp "Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839](/upload/website/prouct_img/small/GTX02839/A4257_IF_01_(1072306)_w_23053123_648.webp "Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839](/upload/website/prouct_img/small/GTX02839/A4257_IHC_01_(1071809)_w_23053123_753.webp "Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Immunoprecipitation (IP). GTX02839](/upload/website/prouct_img/small/GTX02839/CutImage_A4257_IP_01_(1080389)_w_23053123_277.webp "Anti-hnRNP U antibody [GT1242] used in Immunoprecipitation (IP). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839](/upload/website/prouct_img/small/GTX02839/A4257_IHC_03_(1071811)_w_23053123_503.webp "Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839](/upload/website/prouct_img/small/GTX02839/A4257_IF_03_(1072308)_w_23053123_308.webp "Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839](/upload/website/prouct_img/small/GTX02839/CutImage_A4257_WB_01_(1070389)_w_23053123_882.webp "Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839](/upload/website/prouct_img/small/GTX02839/A4257_IHC_02_(1071810)_w_23053123_642.webp "Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839](/upload/website/prouct_img/small/GTX02839/A4257_IF_02_(1072307)_w_23053123_451.webp "Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839](/upload/website/prouct_img/normal/GTX02839/GTX02839_4000000942_20210122_WB_w_23053123_128.webp "Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839](/upload/website/prouct_img/normal/GTX02839/GTX02839_4000000942_20210122_WB_2_w_23053123_816.webp "Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839](/upload/website/prouct_img/normal/GTX02839/A4257_IF_01_(1072306)_w_23053123_648.webp "Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839](/upload/website/prouct_img/normal/GTX02839/A4257_IHC_01_(1071809)_w_23053123_753.webp "Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Immunoprecipitation (IP). GTX02839](/upload/website/prouct_img/normal/GTX02839/CutImage_A4257_IP_01_(1080389)_w_23053123_277.webp "Anti-hnRNP U antibody [GT1242] used in Immunoprecipitation (IP). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839](/upload/website/prouct_img/normal/GTX02839/A4257_IHC_03_(1071811)_w_23053123_503.webp "Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839](/upload/website/prouct_img/normal/GTX02839/A4257_IF_03_(1072308)_w_23053123_308.webp "Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839](/upload/website/prouct_img/normal/GTX02839/CutImage_A4257_WB_01_(1070389)_w_23053123_882.webp "Anti-hnRNP U antibody [GT1242] used in Western Blot (WB). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839](/upload/website/prouct_img/normal/GTX02839/A4257_IHC_02_(1071810)_w_23053123_642.webp "Anti-hnRNP U antibody [GT1242] used in IHC (Paraffin sections) (IHC-P). GTX02839")
![Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839](/upload/website/prouct_img/normal/GTX02839/A4257_IF_02_(1072307)_w_23053123_451.webp "Anti-hnRNP U antibody [GT1242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02839")