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iNOS antibody [N1N2-2], N-term

Anti-iNOS antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124210
Anti-iNOS antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124210
Anti-iNOS antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124210
Anti-iNOS antibody [N1N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124210

Cat. No. GTX124210

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all iNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Chicken, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human iNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162976

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 2 , HEP-NOS , INOS , NOS , NOS2A

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-iNOS antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124210

GTX124210 IHC-P Image

iNOS antibody [N1N2-2], N-term detects iNOS protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
iNOS antibody [N1N2-2], N-term (GTX124210) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124210

GTX124210 IHC-P Image

iNOS antibody [N1N2-2], N-term detects iNOS protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
iNOS antibody [N1N2-2], N-term (GTX124210) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-iNOS antibody [N1N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124210

GTX124210 IHC-P Image

iNOS antibody [N1N2-2], N-term detects iNOS protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
iNOS antibody [N1N2-2], N-term (GTX124210) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-iNOS antibody [N1N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124210

GTX124210 ICC/IF Image

iNOS antibody [N1N2-2], N-term detects iNOS protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: iNOS stained by iNOS antibody [N1N2-2], N-term (GTX124210) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Kuninaka Y et al. Sci Rep 2022; 12 (1) : 20327 Macrophage polarity and wound age determination.
Ozgur T et al. Pak J Med Sci 2013; 29 (2) : 610-3 The expressions of AMACR and iNOS in prostate adenocarcinomas.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169