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iNOS antibody

Anti-iNOS antibody used in Western Blot (WB). GTX127952

Cat No. GTX127952

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

Raw264.7 (50 ng/ml LPS treatment for 4 hr)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nitric oxide synthase 2, inducible , MAC-NOS , NOS-II , Nos-2 , Nos2a , i-NOS , iNOS

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

Database

Research Area

DATA IMAGES

Anti-iNOS antibody used in Western Blot (WB). GTX127952

GTX127952 WB Image

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX127952) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf