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iNOS antibody

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

Cat No. GTX130246

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

Raw264.7 (50 ng/ml LPS treatment for 4 hr)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886221

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nitric oxide synthase 2, inducible , MAC-NOS , NOS-II , Nos-2 , Nos2a , i-NOS , iNOS

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

Database

Research Area

DATA IMAGES

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246

GTX130246 ICC/IF Image

iNOS antibody detects iNOS protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects iNOS protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
iNOS antibody (GTX130246) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody used in Western Blot (WB). GTX130246

GTX130246 WB Image

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX130246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects Inos protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Inos stained by iNOS antibody (GTX130246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

iNOS antibody Cat No. GTX130246

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25105760
Date : Anonymous submitted on 11-Aug-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : Bone marrow derived macrophage
Amount used : 20 μg
Blocking : 3% BSA+0.05% Tween20 in PBS, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Bone marrow derived macrophage (M0) differentiated to M1 by LPS stimulation.
Date : Anonymous submitted on 20-May-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 30μg
Blocking : 5% nonfat dry milk, 28°C, 0.5Hr
Primary Antibody : 1:1000, 28°C, 1Hr
Lane Description : Lane 1: RAW264.7 cells, Lane 2: RAW264.7 cells (1 ug/ml LPS treatment for 24 hours)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf