GeneTex
United States (US)

iNOS antibody

GTX130246_42004_20180329_ICC_IF_R_18121410_396.jpg
GTX130246_42004_20150909_IHC-P_M_18121410_675.jpg
GTX130246_42949_20170818_WB_LPS_18121410_662.jpg

Cat No. GTX130246

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

Raw264.7 (50 ng/ml LPS treatment for 4 hr)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.8mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nos-2 Antibody , Nos2 Antibody , i-NOS Antibody , nitric oxide synthase 2, inducible Antibody , Nos2a Antibody , NOS-II Antibody

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

Database

Research Area

DATA IMAGES
GTX130246_42004_20180329_ICC_IF_R_18121410_396.jpg

GTX130246 ICC/IF Image

iNOS antibody detects iNOS protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX130246_42004_20150909_IHC-P_M_18121410_675.jpg

GTX130246 IHC-P Image

iNOS antibody detects iNOS protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
iNOS antibody (GTX130246) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX130246_42949_20170818_WB_LPS_18121410_662.jpg

GTX130246 WB Image

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX130246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

iNOS antibody Cat No. GTX130246

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-May-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 30μg
Blocking : 5% nonfat dry milk, 28°C, 0.5Hr
Primary Antibody : 1:1000, 28°C, 1Hr
Lane Description : Lane 1: RAW264.7 cells, Lane 2: RAW264.7 cells (1 ug/ml LPS treatment for 24 hours)
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169