GeneTex
United States (US)

iNOS antibody

GTX130246_42004_20180329_ICC_IF_R_18121410_396.jpg
GTX130246_42004_20150909_IHC-P_M_18121410_675.jpg
GTX130246_42949_20170818_WB_LPS_18121410_662.jpg

Cat No. GTX130246

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

Raw264.7 (50 ng/ml LPS treatment for 4 hr)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nitric Oxide Synthase 2, Inducible,Mac-Nos,Nos-Ii,Nos-2,Nos2A,I-Nos,Inos,Nos2

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

Database

Research Area

DATA IMAGES
GTX130246_42004_20180329_ICC_IF_R_18121410_396.jpg

GTX130246 ICC/IF Image

iNOS antibody detects iNOS protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX130246_42004_20150909_IHC-P_M_18121410_675.jpg

GTX130246 IHC-P Image

iNOS antibody detects iNOS protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
iNOS antibody (GTX130246) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX130246_42949_20170818_WB_LPS_18121410_662.jpg

GTX130246 WB Image

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX130246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

iNOS antibody Cat No. GTX130246

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-May-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 30μg
Blocking : 5% nonfat dry milk, 28°C, 0.5Hr
Primary Antibody : 1:1000, 28°C, 1Hr
Lane Description : Lane 1: RAW264.7 cells, Lane 2: RAW264.7 cells (1 ug/ml LPS treatment for 24 hours)
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169