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iNOS antibody

Orthogonal
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246
Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246
Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246

Cat. No. GTX130246

Reference 35
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all iNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886221

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 2, inducible , MAC-NOS , NOS-II , Nos-2 , Nos2a , i-NOS , iNOS

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

Database

Research Area

DATA IMAGES

Anti-iNOS antibody used in Western Blot (WB). GTX130246

GTX130246 WB Image

Orthogonal

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX130246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects iNOS protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects iNOS protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects iNOS protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246

GTX130246 ICC/IF Image

Orthogonal

iNOS antibody detects iNOS protein by immunofluorescent analysis.
Sample: Mock and treated Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246

GTX130246 ICC/IF Image

iNOS antibody detects iNOS protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: iNOS stained by iNOS antibody (GTX130246) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX130246

GTX130246 IHC-P Image

iNOS antibody detects Inos protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Inos stained by iNOS antibody (GTX130246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-iNOS antibody used in Western Blot (WB). GTX130246

GTX130246 WB Image

Orthogonal

The data was published in the journal Front Cell Neurosci in 2018.PMID: 30483062

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130246

GTX130246 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 25105760

REFERENCE

Application Reference

Zhao J et al. J Cell Mol Med 2024; 28 (7) : e18172 The relationship of ALPK1, hyaluronic acid and M1 macrophage polarization in the temporomandibular joint synovitis.
Ishigooka G et al. J Clin Med 2023; 12 (20) Effects of Angiotensin Receptor Blockers on Streptozotocin-Induced Diabetic Cataracts.
Brusini R et al. Pharmaceutics 2023; 15 (7) Assessment of Squalene-Adenosine Nanoparticles in Two Rodent Models of Cardiac Ischemia-Reperfusion.
Teramoto H et al. Cells 2023; 12 (14) Calcineurin B1 Deficiency Reduces Proliferation, Increases Apoptosis, and Alters Secretion in Enteric Glial Cells of Mouse Small Intestine in Culture.
P Wu et al. Mil Med Res 2023; 10 (1) : 35 The marriage of immunomodulatory, angiogenic, and osteogenic capabilities in a piezoelectric hydrogel tissue engineering scaffold for military medicine
Application : ICC/IF
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

iNOS antibody Cat. No. GTX130246

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Date : Anonymous submitted on 02-Nov-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells treated with 1 ug/ml LPS for 24 h
Blocking : Skim milk, RT°C, 1Hr
Primary Antibody : 1:2500, 4°C, 16Hr
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Date : Anonymous submitted on 11-Aug-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : Bone marrow derived macrophage
Amount used : 20 μg
Blocking : 3% BSA+0.05% Tween20 in PBS, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Bone marrow derived macrophage (M0) differentiated to M1 by LPS stimulation.
Anti-iNOS antibody used in Western Blot (WB). GTX130246
Date : Anonymous submitted on 20-May-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 30μg
Blocking : 5% nonfat dry milk, 28°C, 0.5Hr
Primary Antibody : 1:1000, 28°C, 1Hr
Lane Description : Lane 1: RAW264.7 cells, Lane 2: RAW264.7 cells (1 ug/ml LPS treatment for 24 hours)
Submit a Review
Application Reference
Zhao J et al. J Cell Mol Med 2024; 28 (7):e18172 The relationship of ALPK1, hyaluronic acid and M1 macrophage polarization in the temporomandibular joint synovitis.
Ishigooka G et al. J Clin Med 2023; 12 (20) Effects of Angiotensin Receptor Blockers on Streptozotocin-Induced Diabetic Cataracts.
Brusini R et al. Pharmaceutics 2023; 15 (7) Assessment of Squalene-Adenosine Nanoparticles in Two Rodent Models of Cardiac Ischemia-Reperfusion.
Teramoto H et al. Cells 2023; 12 (14) Calcineurin B1 Deficiency Reduces Proliferation, Increases Apoptosis, and Alters Secretion in Enteric Glial Cells of Mouse Small Intestine in Culture.
P Wu et al. Mil Med Res 2023; 10 (1):35 The marriage of immunomodulatory, angiogenic, and osteogenic capabilities in a piezoelectric hydrogel tissue engineering scaffold for military medicine
Application : ICC/IF
Reactivity : Mouse
Zhang J et al. Pharmaceuticals (Basel) 2023; 16 (5) GRK2 Mediates Macrophage Polarization by Regulating EP4-cAMP-pCREB Signaling in Ulcerative Colitis and the Therapeutic Effect of Paroxetine on Mice with DSS-Induced Colitis.
Shi Y et al. J Neuroinflammation 2023; 20 (1):91 N,N-Dimethyl-3β-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1.
Application : WB
Reactivity : Mouse
Huang HC et al. Int J Mol Sci 2022; 23 (13) Effects of PCSK-9 Inhibition by Alirocumab Treatments on Biliary Cirrhotic Rats.
Hsin-Tzu Liu et al. Antioxidants (Basel) 2022; 11 (5) Baicalein Exerts Therapeutic Effects against Endotoxin-Induced Depression-like Behavior in Mice by Decreasing Inflammatory Cytokines and Increasing Brain-Derived Neurotrophic Factor Levels.
Application : WB
Reactivity : Mouse
Lin ZH et al. J Ethnopharmacol 2022; 282:114636 Water extract of medicinal ink (WEMI) attenuates lipopolysaccharide-induced NO production of Raw264.7 cells via downregulating JAK2/STAT3-mediated iNOS expression.
Application : WB
Reactivity : Mouse
Cai M et al. CNS Neurosci Ther 2021; 27 (11):1348-1365 Sevoflurane preconditioning protects experimental ischemic stroke by enhancing anti-inflammatory microglia/macrophages phenotype polarization through GSK-3β/Nrf2 pathway.
Application : ICC/IF
Anyaegbu CC et al. J Neurosci Methods 2021; 359:109223 Simultaneous flow cytometric characterization of multiple cell types and metabolic states in the rat brain after repeated mild traumatic brain injury.
Reactivity : Rat
Ohira H et al. PLoS One 2021; 16 (2):e0246580 Alteration of oxidative-stress and related marker levels in mouse colonic tissues and fecal microbiota structures with chronic ethanol administration: Implications for the pathogenesis of ethanol-related colorectal cancer.
Application : IHC-P
Reactivity : Mouse
Yang Y et al. Biomaterials 2021; 264:120390 Targeted silver nanoparticles for rheumatoid arthritis therapy via macrophage apoptosis and Re-polarization.
Reactivity : Mouse
Tingting Wang et al. Front Cell Dev Biol 2020; 8:593890 DJ-1 Regulates Microglial Polarization Through P62-Mediated TRAF6/IRF5 Signaling in Cerebral Ischemia-Reperfusion.
Application : WB
Reactivity : Rat
Scherwitzl I et al. Mol Ther Oncolytics 2020; 17:431-447 Sindbis Virus with Anti-OX40 Overcomes the Immunosuppressive Tumor Microenvironment of Low-Immunogenic Tumors.
Ekova MR et al. Bull Exp Biol Med 2020; 169 (5):718-720 Peculiarities of the Expression of Inducible NO Synthase in Rat Dentate Gyrus in Depression Modeling.
Application : IHC-P
Reactivity : Rat
Zhou Y et al. Oncol Lett 2020; 20 (6):298 Anti-CD206 antibody-conjugated Fe3O4-based PLGA nanoparticles selectively promote tumor-associated macrophages to polarize to the pro-inflammatory subtype.
Application : WB
Reactivity : Mouse
Zhu S et al. J Agric Food Chem 2020; 68 (42):11726-11734 Collagen Hydrolysate Corrects Anemia in Chronic Kidney Disease via Anti-Inflammatory Renoprotection and HIF-2α-Dependent Erythropoietin and Hepcidin Regulation.
Application : WB
Reactivity : Mouse
Hsu WH et al. Aging (Albany NY) 2020; 12 (11):10556-10577 T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice.
Application : WB
Reactivity : Mouse
Chang JW et al. Cereb Cortex 2020; 30 (8):4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
Application : WB
Reactivity : Rat
Arturo A Wilkins-Rodr?guez et al. Infect Immun 2020; 88 (7) Differential Regulation of l-Arginine Metabolism through Arginase 1 during Infection with Leishmania mexicana Isolates Obtained from Patients with Localized and Diffuse Cutaneous Leishmaniasis.
Application : WB
Reactivity : Mouse
Zhang HK et al. J Mol Neurosci 2020; 70 (4):618-630 Gypenosides Prevent H2O2-Induced Retinal Ganglion Cell Apoptosis by Concurrently Suppressing the Neuronal Oxidative Stress and Inflammatory Response.
Application : WB
Reactivity : Rat
Morita N et al. Microbes Infect 2020; (Epub) Sendai virus V protein decreases nitric oxide production by inhibiting RIG-I signaling in infected RAW264.7 macrophages.
Application : WB
Reactivity : Mouse
Zhang D et al. Nature 2019; 574 (7779):575-580 Metabolic regulation of gene expression by histone lactylation.
Fukuda M et al. J Neurosurg 2019;:1-13 Disruption of P2X4 purinoceptor and suppression of the inflammation associated with cerebral aneurysm formation.
Application : WB
Ghosh R et al. Int J Biol Macromol 2019; 139:221-232 Panax quinquefolius (North American ginseng) cell suspension culture as a source of bioactive polysaccharides: Immunostimulatory activity and characterization of a neutral polysaccharide AGC1.
Application : WB
Reactivity : Mouse
Barati S et al. J Cell Biochem 2019; (Epub) Mesenchymal stem cell mediated effects on microglial phenotype in cuprizone-induced demyelination model.
Application : IHC-P
Reactivity : Mouse
Jiao M et al. Chemosphere 2019; 226:678-686 Effect of the SSeCKS-TRAF6 interaction on gastrodin-mediated protection against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced astrocyte activation and neuronal death.
Application : WB
Reactivity : Mouse
Su WJ et al. Front Cell Neurosci 2018; 12:412 Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.
Application : WB
Reactivity : Mouse
Chao W et al. Int Immunopharmacol 2017; 50:77-86 3, 4-dihydroxybenzalacetone attenuates lipopolysaccharide-induced inflammation in acute lung injury via down-regulation of MMP-2 and MMP-9 activities through suppressing ROS-mediated MAPK and PI3K/AKT signaling pathways.
Application : WB
Reactivity : Mouse
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Mei S et al. PLoS One 2015; 10 (8):e0135867 MicroRNA-200c Promotes Suppressive Potential of Myeloid-Derived Suppressor Cells by Modulating PTEN and FOG2 Expression.
Application : WB
Reactivity : Mouse
Zhao X et al. PLoS One 2014; 9 (8):e104506 PI3K/Akt signaling pathway modulates influenza virus induced mouse alveolar macrophage polarization to M1/M2b.
Application : ICC/IF
Reactivity : Mouse
Yang YC et al. Antioxid Redox Signal 2013; 18 (10):1129-40 Topoisomerase II-mediated DNA cleavage and mutagenesis activated by nitric oxide underlie the inflammation-associated tumorigenesis.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169