GeneTex

  • Country / Location Selection

United States (US)

iNOS antibody

Anti-iNOS antibody used in Western Blot (WB). GTX74171

Cat. No. GTX74171

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
50 μl ($399)
See all iNOS products

APPLICATION

Application Note

For IHC-Fr: Use at a dilution of 1:500. For WB: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

131 kDa. ( Note )

Product Note

Recognizes inducible nitric oxide sythase (iNOS), and does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Mouse macrophage NOS C-terminal peptide (1131-1144) + additional N-terminal Cys conjugated to KLH (CKKGSALEEPKATRL).

Purification

Unpurified
From polyclonal serum.

Conjugation

Unconjugated

RRID

AB_378203

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 2, inducible , MAC-NOS , NOS-II , Nos-2 , Nos2a , i-NOS , iNOS

Cellular Localization

Cytoplasm, cytosol

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-iNOS antibody used in Western Blot (WB). GTX74171

GTX74171 WB Image

Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Mouse macrophage RAW 264.7 cell line induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase (GTX74171)

REFERENCE

Application Reference

Lin J et al. Heliyon 2023; 9 (5) : e15689 Application of silver nanoparticles for improving motor recovery after spinal cord injury via reduction of pro-inflammatory M1 macrophages.
Lin ZH et al. Int J Biol Macromol 2023; 241 : 124648 GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway.
Wang Q et al. Front Immunol 2020; 11 : 846 Perforin Acts as an Immune Regulator to Prevent the Progression of NAFLD.
Omar NM et al. Acta Histochem 2017; 119 (2) : 161-171 The possible protective role of pumpkin seed oil in an animal model of acid aspiration pneumonia: Light and electron microscopic study.
Application : IHC-P
Reactivity : Rat
Submit a Reference

REVIEW

There are currently no reviews for iNOS antibody (GTX74171). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399