lacto-N-fucopentaose I antibody [R-17F]

R-17F is reported to exhibit potent dose-dependent cytotoxicity when it is added to living human iPS/ES cells.
[Left] After the incubation of iPS cell suspension with R-17F at 4ºC for only 45 minutes, the percentage of viable cells decreased concentration-dependently (red triangles). Blue squares : effects of the isotype (IgG1)-matching control antibody (anti-α-MBP) as Negative Control.
[Right] When R-17F-treated iPS cells were incubated with a small amount (0.025-0.1 μg) of the secondary antibody (goat anti-mouse IgG1 antibody), the cytotoxic effect of R-17F was enhanced significantly in a dose-dependent manner (red triangles).

WB analysis of human iPS and HEK293 cell lysate using GTX57213 lacto-N-fucopentaose I antibody [R-17F].
One major positive band and several minor bands were specific to human iPS cells, and any positive band was not obtained with HEK293 cells.
Human iPS cell: LNFP I positive
HEK293 cell lysate: Negative Control
Loading : 5ug per lane
Dilution : 1:2000

Cultured human iPS cells were stained with R-17F, SSEA-3, and SSEA-4 antibodies. [bars: 100μm]
R-17F stained the entire surface of the cell membranes equally, while the staining by SSEA-3 and SSEA-4 antibodies are not evenly. This suggests that R-17F epitope is expressed ubiquitously all over the human iPS cells.