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lacto-N-fucopentaose I antibody [R-17F]

Anti-lacto-N-fucopentaose I antibody [R-17F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57213
Anti-lacto-N-fucopentaose I antibody [R-17F] used in Western Blot (WB). GTX57213
Anti-lacto-N-fucopentaose I antibody [R-17F] used in (). GTX57213
Anti-lacto-N-fucopentaose I antibody [R-17F] used in Functional Assay (Functional Assay). GTX57213

Cat. No. GTX57213

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 R-17F

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, Functional Assay

Reactivity

 Human
Package
100 μl ($409)
See all lacto-N-fucopentaose I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF Assay dependent
FACS Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

Product Note

This antibody recognizes lacto-N-fucopentaose I (LNFP I: Fucα1–2Galβ1–3GlcNAcβ1–3Galβ1–4Glc) on a glycolipid / glycoprotein. R-17F epitopes are expressed on undifferentiated human induced pluripotent stem (iPS) / embryonic stem (ES) cells but not on human embryonal carcinoma (EC) cells nor on differentiated human iPS/ES cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human iPS cell line, Tic, derived from human fetus lung cells.

Purification

Protein A Purified.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-lacto-N-fucopentaose I antibody [R-17F] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57213

GTX57213 ICC/IF Image

Cultured human iPS cells were stained with R-17F, SSEA-3, and SSEA-4 antibodies. [bars: 100μm]
R-17F stained the entire surface of the cell membranes equally, while the staining by SSEA-3 and SSEA-4 antibodies are not evenly. This suggests that R-17F epitope is expressed ubiquitously all over the human iPS cells.

Anti-lacto-N-fucopentaose I antibody [R-17F] used in Western Blot (WB). GTX57213

GTX57213 WB Image

WB analysis of human iPS and HEK293 cell lysate using GTX57213 lacto-N-fucopentaose I antibody [R-17F].
One major positive band and several minor bands were specific to human iPS cells, and any positive band was not obtained with HEK293 cells.
Human iPS cell: LNFP I positive
HEK293 cell lysate: Negative Control
Loading : 5ug per lane
Dilution : 1:2000

Anti-lacto-N-fucopentaose I antibody [R-17F] used in (). GTX57213

GTX57213 Image

Anti-lacto-N-fucopentaose I antibody [R-17F] used in Functional Assay (Functional Assay). GTX57213

GTX57213 Functional Assay Image

R-17F is reported to exhibit potent dose-dependent cytotoxicity when it is added to living human iPS/ES cells.
[Left] After the incubation of iPS cell suspension with R-17F at 4ºC for only 45 minutes, the percentage of viable cells decreased concentration-dependently (red triangles). Blue squares : effects of the isotype (IgG1)-matching control antibody (anti-α-MBP) as Negative Control.
[Right] When R-17F-treated iPS cells were incubated with a small amount (0.025-0.1 μg) of the secondary antibody (goat anti-mouse IgG1 antibody), the cytotoxic effect of R-17F was enhanced significantly in a dose-dependent manner (red triangles).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 409