Unboiled rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with mAChR M1 antibody [HL3933] (GTX642178) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with mAChR M1 antibody [HL3933] (GTX642178) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.