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mAChR M2 antibody [31-1D1]

Anti-mAChR M2 antibody [31-1D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22805
Anti-mAChR M2 antibody [31-1D1] used in IHC (Paraffin sections) (IHC-P). GTX22805

Cat. No. GTX22805

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 31-1D1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($399)
See all mAChR M2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 2μg/ml
IHC-P 1:20
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Product Note

This antibody is specific for the m2 mAChR subtype.

PROPERTIES

Form

Liquid

Buffer

Buffer, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Purified porcine cardiac m2 muscarinic acetylcholine receptor.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_369488

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cholinergic receptor muscarinic 2

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-mAChR M2 antibody [31-1D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22805

GTX22805 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX22805 mAChR M2 antibody [31-1D1]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-mAChR M2 antibody [31-1D1] used in IHC (Paraffin sections) (IHC-P). GTX22805

GTX22805 IHC-P Image

IHC-P analysis of human kidney tissue using GTX22805 mAChR M2 antibody [31-1D1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

REFERENCE

Application Reference

Chen SF et al. J Formos Med Assoc 2018; (Epub)
Application : WB
Reactivity : Human
Jiang YH et al. J Urol 2016; Urothelial Barrier Deficits, Suburothelial Inflammation and Altered Sensory Protein Expressions in Detrusor Underactivity.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399