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mAChR M2 antibody [GT1272]

Recombinant antibody
Anti-mAChR M2 antibody [GT1272] used in Western Blot (WB). GTX03184
Anti-mAChR M2 antibody [GT1272] used in IHC (Paraffin sections) (IHC-P). GTX03184
Anti-mAChR M2 antibody [GT1272] used in Western Blot (WB). GTX03184
Anti-mAChR M2 antibody [GT1272] used in IHC (Paraffin sections) (IHC-P). GTX03184
Anti-mAChR M2 antibody [GT1272] used in IHC (Paraffin sections) (IHC-P). GTX03184

Cat. No. GTX03184

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1272

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all mAChR M2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ACM2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cholinergic receptor muscarinic 2 , HM2

Cellular Localization

Cell membrane

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-mAChR M2 antibody [GT1272] used in Western Blot (WB). GTX03184

GTX03184 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with mAChR M2 antibody [GT1272] (GTX03184) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-mAChR M2 antibody [GT1272] used in IHC (Paraffin sections) (IHC-P). GTX03184

GTX03184 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX03184 mAChR M2 antibody [GT1272].
Dilution : 1:100

Anti-mAChR M2 antibody [GT1272] used in Western Blot (WB). GTX03184

GTX03184 WB Image

WB analysis of various samples using GTX03184 mAChR M2 antibody [GT1272].
Dilution : 1:1000
Loading : 25μg per lane

Anti-mAChR M2 antibody [GT1272] used in IHC (Paraffin sections) (IHC-P). GTX03184

GTX03184 IHC-P Image

IHC-P analysis of human esophageal cancer section using GTX03184 mAChR M2 antibody [GT1272].
Dilution : 1:100

Anti-mAChR M2 antibody [GT1272] used in IHC (Paraffin sections) (IHC-P). GTX03184

GTX03184 IHC-P Image

IHC-P analysis of rat testis tissue section using GTX03184 mAChR M2 antibody [GT1272].
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 439