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mAChR M3 antibody

Anti-mAChR M3 antibody used in Western Blot (WB). GTX111637
Anti-mAChR M3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111637
Anti-mAChR M3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111637

Cat No. GTX111637

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, FACS

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Rat, Bovine, Guinea pig, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of Human mAChR M3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cholinergic receptor muscarinic 3 , EGBRS , HM3 , PBS

Cellular Localization

Cell membrane , Cell junction , synapse , postsynaptic cell membrane , Basolateral cell membrane

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-mAChR M3 antibody used in Western Blot (WB). GTX111637

GTX111637 WB Image

mAChR M3 antibody detects mAChR M3 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with mAChR M3 antibody (GTX111637) at a dilution of 1:1000.

Anti-mAChR M3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111637

GTX111637 ICC/IF Image

mAChR M3 antibody detects mAChR M3 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: mAChR M3 stained by mAChR M3 antibody (GTX111637) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-mAChR M3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111637

GTX111637 ICC/IF Image

mAChR M3 antibody detects mAChR M3 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: mAChR M3 stained by mAChR M3 antibody (GTX111637) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169