GeneTex
United States (US)

mAChR M5 antibody

GTX53955_20180530_IHC-P_18121410_719.jpg
GTX53955_20180530_ICCIF_18121410_234.jpg
GTX53955_20180530_WB_18121410_423.jpg

Cat No. GTX53955

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

60 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 230-430 of human CHRM5 (NP_036257.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CHRM5 Antibody , HM5 Antibody

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX53955_20180530_IHC-P_18121410_719.jpg

GTX53955 IHC-P Image

IHC-P analysis of rat brain tissue using GTX53955 mAChR M5 antibody.
Dilution : 1:100

GTX53955_20180530_ICCIF_18121410_234.jpg

GTX53955 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX53955 mAChR M5 antibody.
Blue : DAPI

GTX53955_20180530_WB_18121410_423.jpg

GTX53955 WB Image

WB analysis of various samples using GTX53955 mAChR M5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 319