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mCherry antibody [GT844]

Anti-mCherry antibody [GT844] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630195
Anti-mCherry antibody [GT844] used in Western Blot (WB). GTX630195

Cat. No. GTX630195

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT844

Isotype

 IgG2a

Application

 WB, ICC/IF
Package
100 μl ($399),
25 μl ($169)
See all mCherry products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

mCherry-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length mCherry recombinant protein

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888197

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mCherry (full length)

Research Area

DATA IMAGES

Anti-mCherry antibody [GT844] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630195

GTX630195 ICC/IF Image

mCherry antibody [GT844] detects mCherry protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mCherry stained by mCherry antibody [GT844] (GTX630195) diluted at 1:1000.
Red: mCherry is expressed in the transfected cell.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-mCherry antibody [GT844] used in Western Blot (WB). GTX630195

GTX630195 WB Image

mCherry antibody [GT844] detects mCherry protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of mCherry-tagged protein expressing 293T cells
5 % SDS-PAGE
mCherry antibody [GT844] (GTX630195) dilution: 1:1000

REFERENCE

Application Reference

Zhou JR et al. Parasit Vectors 2020; 13 (1) : 40 Hc-hrg-2, a glutathione transferase gene, regulates heme homeostasis in the blood-feeding parasitic nematode Haemonchus contortus.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations