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mCherry antibody [GT857]

Anti-mCherry antibody [GT857] used in Western Blot (WB). GTX630189

Cat. No. GTX630189

Reference 3
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT857

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Wm, IHC

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all mCherry products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-Wm Assay dependent
IHC Assay dependent
Not tested in other applications.

Positive Control

mCherry-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length mCherry recombinant protein

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888193

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mCherry (full length)

Research Area

DATA IMAGES

Anti-mCherry antibody [GT857] used in Western Blot (WB). GTX630189

GTX630189 WB Image

mCherry antibody [GT857] detects mCherry protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of mCherry-tagged protein expressing 293T cells
5% SDS-PAGE
mCherry antibody [GT857] (GTX630189) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wu CY et al. iScience 2021; 24 (12) : 103437 Dihydroceramide desaturase promotes the formation of intraluminal vesicles and inhibits autophagy to increase exosome production.
Numata T et al. Commun Biol 2021; 4 (1) : 599 TRPM7 is an essential regulator for volume-sensitive outwardly rectifying anion channel.
Application : WB, ICC/IF
Shukla S et al. Methods Mol Biol 2018; 1776 : 61-84 In Planta Production of Fluorescent Filamentous Plant Virus-Based Nanoparticles.
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REVIEW

mCherry antibody [GT857] Cat. No. GTX630189

Rating
( Average 5 based on 3 users reviews)
Application
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 2 users reviews)
Anti-mCherry antibody [GT857] used in IHC (Whole mount) (IHC-Wm). GTX630189
Date : Anonymous submitted on 01-Jun-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila eye
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 10 minHr
Primary Antibody : 1:200, 4°C, 16Hr
Anti-mCherry antibody [GT857] used in Immunohistochemistry (IHC). GTX630189
Date : Anonymous submitted on 22-Feb-2018
Score :
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Red: GTX630189 mcherry antibody: medulla, Green: GTX113617 GFP antibody: photoreceptor cell R8
Anti-mCherry antibody [GT857] used in Immunohistochemistry (IHC). GTX630189
Date : Anonymous submitted on 18-Jul-2017
Score :
Application Tested : IHC
Sample : Drosophila Brain tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : red: mcherry 543 nm (overexpression) ; megenta: anti-mouse with cy5
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations