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mCherry antibody [GT857]

Anti-mCherry antibody [GT857] used in Western Blot (WB). GTX630189

Cat No. GTX630189

Host Mouse
Clonality Monoclonal
Clone Name GT857
Isotype IgG1
Application WB, IHC-Wm, IHC
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Wm Assay dependent
IHC Assay dependent
Not tested in other applications.

Positive Control

mCherry-tagged protein expressing 293T
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length mCherry recombinant protein

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

mCherry (full length)

Research Area

DATA IMAGES
Anti-mCherry antibody [GT857] used in Western Blot (WB). GTX630189

GTX630189 WB Image

mCherry antibody [GT857] detects mCherry protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of mCherry-tagged protein expressing 293T cells
5% SDS-PAGE
mCherry antibody [GT857] (GTX630189) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

mCherry antibody [GT857] Cat No. GTX630189

Rating ( Average 5 based on 3 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 01-Jun-2018
Score :
Application Tested : IHC-Wm
Sample : Drosophila eye
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 10 minHr
Primary Antibody : 1:200, 4°C, 16Hr
Date : Anonymous submitted on 22-Feb-2018
Score :
Application Tested : IHC
Sample : Drosophila Retinal tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Red: GTX630189 mcherry antibody: medulla, Green: GTX113617 GFP antibody: photoreceptor cell R8
Date : Anonymous submitted on 18-Jul-2017
Score :
Application Tested : IHC
Sample : Drosophila Brain tissue
Blocking : 10% NGS in 0.3% PBST (triton-X-100), 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : red: mcherry 543 nm (overexpression) ; megenta: anti-mouse with cy5
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169